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Purification of an Aspartic Proteinase from Aspergillus Aculeatus

  • Steen B. Mortensen
  • Claus Dambmann
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 306)

Abstract

Aspergilli are known to produce a number of extracellular enzymes (e.g. proteinases, carbohydrases, lipases). Several are utilized in commercial products, based on a main activity, but often containing minor side activities. Usually it is difficult to isolate small amounts of a side activity, but in such cases affinity chromatography can be a valuable tool, due to its specific action. We have succeeded in isolating a proteolytic side activity from the Aspergillus aculeatus derived carbohydrase product Viscozyme™ (Novo Nordisk A/S, Denmark). The proteinase was isolated by affinity chromatography on a bacitracin matrix, and was shown to be an aspartic proteinase.

Keywords

Sodium Acetate Affinity Chromatography Aspartic Proteinase Side Activity Aspergillus Aculeatus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Steen B. Mortensen
    • 1
  • Claus Dambmann
    • 1
  1. 1.Bioscience and Industrial BiotechnologyNovo Nordisk A/SBagsvaerdDenmark

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