Purification of an Aspartic Proteinase from Aspergillus Aculeatus

  • Steen B. Mortensen
  • Claus Dambmann
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 306)


Aspergilli are known to produce a number of extracellular enzymes (e.g. proteinases, carbohydrases, lipases). Several are utilized in commercial products, based on a main activity, but often containing minor side activities. Usually it is difficult to isolate small amounts of a side activity, but in such cases affinity chromatography can be a valuable tool, due to its specific action. We have succeeded in isolating a proteolytic side activity from the Aspergillus aculeatus derived carbohydrase product Viscozyme™ (Novo Nordisk A/S, Denmark). The proteinase was isolated by affinity chromatography on a bacitracin matrix, and was shown to be an aspartic proteinase.


Sodium Acetate Affinity Chromatography Aspartic Proteinase Side Activity Aspergillus Aculeatus 
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  1. Davidson, R., Gertler, A. & Hofmann, T., 1975, Biochem. J. 147: 45–53.PubMedGoogle Scholar
  2. Mortensen, S. B., Thim, L., Christensen, T., Wldike, H. F., Boel, E., Hjortsh, J. K. & Hansen, M. T., 1989, J. Chromatogr. 476: 227–233.CrossRefGoogle Scholar
  3. Stepanov, V. M. & Rudenskaya, G. N., 1983, J. Appl. Biochm 5: 420.Google Scholar
  4. Stepanov, V. M., Rudenskaya, G. N., Garida, A. V. & Osterman, A. I., 1981, J. Biochem. Biophys. 5: 177.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Steen B. Mortensen
    • 1
  • Claus Dambmann
    • 1
  1. 1.Bioscience and Industrial BiotechnologyNovo Nordisk A/SBagsvaerdDenmark

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