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Thermopsin, A Thermostable Acid Protease from Sulfolobus Acidocaldarius

  • Xinli Lin
  • Martin Fusek
  • Jordan Tang
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 306)

Abstract

Most of the well-studied aspartic proteases, including those derived from yeast, fungi, plants and animal sources, are stable in temperatures up to about 50° to 60°C. Aspartic proteases which can function at high temperature in the range of 80° to 100°C have not been reported so far. We searched for thermostable acid proteases in the thermoacidophilic archaebacteria, Sulfolobus acidocaldarius, Sulfolobus solfataricus, and Thermoplasma acidophilum, because these organisms grow best in acidic media in a pH near 2 and at temperature of 80°C. Using a highly sensitive radioassay (Lin et al, 1989), we found proteolytic activities in the cultures of all three bacteria. The highest activity was found in Sulfolobus acidocaldarius. This protease was named thermopsin.

Keywords

Aspartic Protease Acid Protease High Substrate Concentration Sodium Formate Iodoacetic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Fusek, M., Lin, X. & J. Tang, 1990, J. Biol. Chem. 265: 1496–1501.PubMedGoogle Scholar
  2. Lin, X., Wong, R. N. S. & Tang, J., 1989, J. Biol. Chem. 264: 4482–4489.PubMedGoogle Scholar
  3. Lin, X. & Tang J., 1990, J. Biol. Chem. 265: 1490–95.PubMedGoogle Scholar
  4. Maita, T., Nagata, S., Matsuda, G., Oda, K., Murao, S. & Tsura, D., 1984, J. Biochem. 95: 465–475.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Xinli Lin
    • 1
  • Martin Fusek
    • 1
  • Jordan Tang
    • 1
  1. 1.Laboratory of Protein StudiesOklahoma Medical Research FoundationOklahoma CityUSA

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