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Structure-Function Database for Active Site Binding to the Aspartic Proteinases

  • Chetana Rao
  • Paula E. Scarborough
  • W. Todd Lowther
  • John Kay
  • Brian Batley
  • Stephen Rapundalo
  • Sylvester Klutchko
  • Michael D. Taylor
  • Ben M. Dunn
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 306)

Abstract

Aspartic Proteinases are produced in the human body by a variety of cells. Some of these proteins, for example, pepsin, gastricsin and renin are secreted and exert their effects in the extracellular spaces. Cathepsin D and cathepsin E, on the other hand, are intracellular enzymes.

Keywords

Chromogenic Substrate Aspartic Proteinase Parent Peptide Human Cathepsin Side Chain Interaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Abad-Zapatero, C., Rydel, T. J., Neidhart, D. J., Luly, J. and Erickson, J. W., Inhibitor binding induces structural changes in porcine pepsin, in: “Structure and Function of the Aspartic Proteinases: Genetics, Structures and Mechanisms,” Ben M. Dunn, ed., Plenum Press, New York.Google Scholar
  2. Afting, E. G. and Becker, M. L., 1981, Two-step affinity-chromatographic purification of cathepsin D from pig myometrium with high yield, Biochem. J. 197: 519–522.PubMedGoogle Scholar
  3. Dunn, B. M, Jiminez, M, Parten, B. F., Valier, M. J., Rolph, C. E. and Kay, J., 1986, A systemic series of synthetic chromophoric substrates for aspartic proteinases, Biochem. J. 237: 899–906.PubMedGoogle Scholar
  4. Pohl, J. and Dunn, B. M., 1988, Secondary enzyme-substrate interactions: Kinetic evidence for ionic interactions between substrate side chains and the pepsin active site, Biochemistry 27: 4827–4834.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Chetana Rao
    • 1
  • Paula E. Scarborough
    • 1
  • W. Todd Lowther
    • 1
  • John Kay
    • 2
  • Brian Batley
    • 3
  • Stephen Rapundalo
    • 3
  • Sylvester Klutchko
    • 3
  • Michael D. Taylor
    • 3
  • Ben M. Dunn
    • 1
  1. 1.Department of Biochemistry & Molecular BiologyUniversity of FloridaGainesvilleUSA
  2. 2.College of CardiffUniversity of WalesWalesUK
  3. 3.Parke-Davis Pharmaceutical Research DivisionWarner-Lambert CompanyAnn ArborUSA

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