A Highly Informative Polymorphism of the Pepsinogen C Gene Detected by Polymerase Chain Reaction

  • R. Thomas Taggart
  • Takeshi Azuma
  • S. Wu
  • Graeme I. Bell
  • Anne M. Bowcock
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 306)


Human pepsinogen, the inactive precursor of pepsin, comprises two biochemically and immunologically distinct groups of isozymogens; namely PGA and PGC. PGA has been localized to 11ql3 and PGC to 6p21.1→pter.1–4 Previous studies demonstrated that a region of the human pepsinogen C gene contained a restriction fragment length polymorphism by Southern blot analysis of genomic DNA with a PGC cDNA clone. This RFLP involved a 100 bp insertion or deletion of intron sequence located between exons 7 and 8. Analysis of families segregating for this polymorphism indicated that there is a single human PGC gene located on the short arm of chromosome 6.


Southern Analysis Polymorphic Region Restriction Fragment Polymorphism Informative Polymorphism Conserve Linkage Group 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    R. T. Taggart, T. K. Mohandas, T. B. Shows and G. I. Bell, A gene complex containing variable numbers of pepsinogen genes is located in the centromeric region of human chromosome 11 and determines the high frequency electrophoretic polymorphism, Proc. Natl. Acad. Sci. U. S. A. 82: 6240–6244 (1985).PubMedCrossRefGoogle Scholar
  2. 2.
    H. Nakai, M. G. Byers, T. B. Shows and R. T. Taggart, Assignment of the pepsinogen gene complex (PGA) to human chromosome 11ql3 by in situ hybridization, Cytogenet Cell Genet 43: 215–217 (1986).PubMedCrossRefGoogle Scholar
  3. 3.
    R. T. Taggart, L. G. Cass, T. K. Mohandas, P. Derby, P. J. Barr, G. Pals and G. I. Bell, Human pepsinogen C (progastricsin): Isolation of cDNA clones, localization to chromosome 6, and sequence homology with pepsinogen A, J Biol Chem 264: 375–379 (1989).PubMedGoogle Scholar
  4. 4.
    G. Pals, T. Azuma, T. K. Mohandas, G. I. Bell, J. A. Bacon, I. M. Samloff, D. A. Walz, P. J. Barr and R. T. Taggart, Human pepsinogen C (progastricsin) polymorphism: Evidence for a single locus located at 6p21.1-pter, Genomics 4: 137–145 (1989).PubMedCrossRefGoogle Scholar
  5. 5.
    T. Azuma, G. Pals and R. T. Taggart, RFLP for the human pepsinogen C gene (PGC), Nucleic Acids Res 16: 9372 (1988).PubMedCrossRefGoogle Scholar
  6. 6.
    A. P. Feinberg and B. Vogelstein, A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity, Analyt Biochem 132: 6–13 (1983).PubMedCrossRefGoogle Scholar
  7. 7.
    J. E. Arrand, Preparation of nucleic acid probes, in: “Nucleic Acid Hybridization,” B. D. Hames and S. J. Higgins, eds pp. 17–44, IRL Press, Oxford, (1985).Google Scholar
  8. 8.
    A. M. Bowcock, A. Ray, H. Erlich and P. B. Sehgal, Rapid detection and sequencing of alleles in the 3′ flanking region of the interleukin-6 gene, Nucleic Acids Res. 17: 6855–6864 (1989).PubMedCrossRefGoogle Scholar
  9. 9.
    J. W. Schumm, R. G. Knowlton, J. G. Braman, D. F. Barker, D. Botstein, G. Akots, V. A. Brown, T. A. Gravius, C. Helms, K. Hsiao, K. Rediker, J. G. Thurston, and H. Donnis-Keller, Identification of more than 500 RFLPs by screening random genomic clones, Am. J. Hum. Genet. 42: 143–159 (1988).PubMedGoogle Scholar
  10. 10.
    J. M. Szymura and J. Klein, Linkage of a gene controlling urinary pepsinogen with the major histocompatibility complex of the mouse, Immunogenet. 13: 267–271 (1981).CrossRefGoogle Scholar
  11. 11.
    A. G. Searle, J. Peters, M. F. Lyon, E. P. Evans, J. H. Edwards and V. J. Buckle, Chromosome Maps of Man and Mouse, III, Genomics 1: 3–18 (1987).PubMedCrossRefGoogle Scholar
  12. 12.
    U. B. Gyllensten and H. D. Erlich, Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus, Proc. Natl. Acad. Sci. U.S.A. 85: 7652–7656 (1988).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • R. Thomas Taggart
    • 1
  • Takeshi Azuma
    • 1
    • 2
  • S. Wu
    • 3
  • Graeme I. Bell
    • 3
  • Anne M. Bowcock
    • 4
  1. 1.Department of Molecular Biology and GeneticsWayne State University School of MedicineDetroitUSA
  2. 2.Department of Preventive MedicineKyoto Prefectural UniversityKyotoJapan
  3. 3.Howard Hughes Medical InstituteThe University of ChicagoChicagoUSA
  4. 4.Department of PediatricsThe University of Texas, Southwestern Medical CenterDallasUSA

Personalised recommendations