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A Highly Informative Polymorphism of the Pepsinogen C Gene Detected by Polymerase Chain Reaction

  • R. Thomas Taggart
  • Takeshi Azuma
  • S. Wu
  • Graeme I. Bell
  • Anne M. Bowcock
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 306)

Abstract

Human pepsinogen, the inactive precursor of pepsin, comprises two biochemically and immunologically distinct groups of isozymogens; namely PGA and PGC. PGA has been localized to 11ql3 and PGC to 6p21.1→pter.1–4 Previous studies demonstrated that a region of the human pepsinogen C gene contained a restriction fragment length polymorphism by Southern blot analysis of genomic DNA with a PGC cDNA clone. This RFLP involved a 100 bp insertion or deletion of intron sequence located between exons 7 and 8. Analysis of families segregating for this polymorphism indicated that there is a single human PGC gene located on the short arm of chromosome 6.

Keywords

Southern Analysis Polymorphic Region Restriction Fragment Polymorphism Informative Polymorphism Conserve Linkage Group 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • R. Thomas Taggart
    • 1
  • Takeshi Azuma
    • 1
    • 2
  • S. Wu
    • 3
  • Graeme I. Bell
    • 3
  • Anne M. Bowcock
    • 4
  1. 1.Department of Molecular Biology and GeneticsWayne State University School of MedicineDetroitUSA
  2. 2.Department of Preventive MedicineKyoto Prefectural UniversityKyotoJapan
  3. 3.Howard Hughes Medical InstituteThe University of ChicagoChicagoUSA
  4. 4.Department of PediatricsThe University of Texas, Southwestern Medical CenterDallasUSA

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