A Highly Informative Polymorphism of the Pepsinogen C Gene Detected by Polymerase Chain Reaction
Human pepsinogen, the inactive precursor of pepsin, comprises two biochemically and immunologically distinct groups of isozymogens; namely PGA and PGC. PGA has been localized to 11ql3 and PGC to 6p21.1→pter.1–4 Previous studies demonstrated that a region of the human pepsinogen C gene contained a restriction fragment length polymorphism by Southern blot analysis of genomic DNA with a PGC cDNA clone. This RFLP involved a 100 bp insertion or deletion of intron sequence located between exons 7 and 8. Analysis of families segregating for this polymorphism indicated that there is a single human PGC gene located on the short arm of chromosome 6.
KeywordsSouthern Analysis Polymorphic Region Restriction Fragment Polymorphism Informative Polymorphism Conserve Linkage Group
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