Biological Aspects of Monocyte Chemoattractant Protein-1 (MCP-1)

  • Edward J. Leonard
  • Alison Skeel
  • Teizo Yoshimura
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 305)


In 1973, Altman et al. published a paper entitled “A human mononuclear leukocyte chemotactic factor: characterization, specificity and kinetics of production by homologous leukocytes” (1). They reported that stimulation by tuberculin (PPD) of blood mononuclear leukocytes from individuals with a positive skin test to tuberculin caused production of a chemotactic factor for human monocytes. Since addition of PPD to leucocytes from PPD-negative subjects did not elicit the factor, the authors suggested that it was an in vitro correlate of delayed hypersensitivity and could account for recruitment of macrophages in cellular immune reactions. The factor was a heat stable macromolecule with a molecular mass, estimated by gel filtration, of about 12,500 daltons. Altman later referred to this molecule as leukocyte-derived chemotactic factor, or LDCF (2). In 1989, we reported the purification to homogeneity of the predominant chemotactic activity for monocytes in culture fluids of PHA-stimulated human mononuclear leukocytes (3). We suggested that this 8700 dalton protein, called MCP-1, is the attractant studied by Altman et al. fifteen years earlier. The present communication addresses the possible role of MCP-1 in cellular immunity. Other biological aspects of MCP-1 were recently reviewed (4).


Culture Fluid Allergic Contact Dermatitis Chemotactic Activity Positive Skin Test Mononuclear Leukocyte 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Altman, L.C., R. Snyderman, J.J. Oppenheim, and S.E. Mergenhagen. 1973. A human mononuclear leukocyte chemotactic factor: characterization, specificity and kinetics of production by homologous leukocytes. J. Immunol. 110: 801–810.PubMedGoogle Scholar
  2. 2.
    Altman, L.C. 1978. Chemotactic lymphokines: a review. In Leukocyte Chemotaxis. J.I. Gallin and P.G. Quie. eds. Raven Press. New York. p 267.Google Scholar
  3. 3.
    Yoshimura, T., E.A. Robinson, S. Tanaka, E. Appella, and E.J. Leonard. 1989. Purification and amino acid analysis of two human monocyte chemoattractants produced by phytohemagglutinin-stimulated human blood mononuclear leukocytes. J. Immunol. 142: 1956–1962.PubMedGoogle Scholar
  4. 4.
    Leonard, E.J. and T. Yoshimura. 1990. Human monocyte chemoattractant protein-1 (MCP-1). Immunology Today 11: 97–101.PubMedCrossRefGoogle Scholar
  5. 5.
    Dvorak, H.F. and M.C. Mihm, jr. 1972. Basophil leukocytes in allergic contact dermatitis. J. Exp Med. 135: 235.PubMedCrossRefGoogle Scholar
  6. 6.
    Askenase, P.W. 1977. Role of basophils, mast cells and vasoamines in hypersensitivity reactions with a delayed time course. Prog. Allergy 23: 199–320.PubMedGoogle Scholar
  7. 7.
    Leonard, E.J., M.A. Lett-Brown, and P.W. Askenase. 1979. Simultaneous generation of tuberculin type and cutaneous basophilic hypersensitivity at separate sites in the guinea pig. Int. Arch. Allergy Appl. Immuno. 58: 460–469.CrossRefGoogle Scholar
  8. 8.
    Leonard, E.J., T. Yoshimura, S. Tanaka, and M. Raffeld. 1990. Neutrophil recruitment by intradermally injected neutrophil attractant/activating protein-1 (NAP-1) in human subjects. In Pathophysiologic and therapeutic roles of cytokines. Dinarello, C.A., Kluger, M., Powanda, M. and Oppenheim, J.J. eds. Alan Liss, New York. In press.Google Scholar
  9. 9.
    Williams, T.J. and P.J. Jose. 1981. Mediation of increased vascular permeability after complement activation. Histamine-independent action of rabbit C5a. J. Exp. Med. 153: 136–153.PubMedCrossRefGoogle Scholar
  10. 10.
    El-Naggar, A.L., D.E. van Epps, and R.C. Williams, Jr. 1981. Effect of culturing on the human locomotor response to casein, C5a, and f-Met-Leu-Phe. Cell Immunol. 60: 43.PubMedCrossRefGoogle Scholar
  11. 11.
    Leonard, E.J. 1987. Two populations of human blood basophils: effect of prednisone on circulating numbers. J. Allergy Clin. Immunol. 79: 775–780.PubMedCrossRefGoogle Scholar
  12. 12.
    Kurimoto, Y., A.L. de Weck, and C.A. Dahinden. 1989. Interleukin 3-dependent mediator release in basophils triggered by C5a. J. Exp. Med. 170: 467–479.PubMedCrossRefGoogle Scholar
  13. 13.
    Dahinden, C.A., Y. Kurimoto, A.L. deWeck, I. Lindley, B. Dewald, and M. Baggiolini. 1989. The neutrophil-activating peptide NAF/NAP-1 induces histamine and leukotriene release by interleukin 3-primed basophils. J. Exp. Med. 170: 1787–1792.PubMedCrossRefGoogle Scholar
  14. 14.
    Leonard, E.J. and T. Yoshimura. 1990. Neutrophil attractant/activation protein-1 (NAP-1 [Interleukin-8]). Am. J. Respir. Cell Mol. Biol. 2: 479–486.PubMedGoogle Scholar
  15. 15.
    Sylvester, I., J.A. Rankin, T. Yoshimura, S. Tanaka, and E.J. Leonard. 1990. Secretion of neutrophil attractant activation protein by LP-stimulated lung macrophages determined by both enzyme-linked immunosorbent assay and N-terminal sequence analysis. Am. Rev. Resp. Dis. 141: 683–688.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Edward J. Leonard
    • 1
  • Alison Skeel
    • 1
  • Teizo Yoshimura
    • 1
  1. 1.Immunopathology Section Laboratory of ImmunobiologyNational Cancer InstituteFrederickUSA

Personalised recommendations