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Cloning Phospholamban cDNA from Rat Aortic Smooth Muscle

  • Kwang S. Hwang
  • Bernardo Nadal-Ginard
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 304)

Abstract

Phospholamban (PLN, Kirchberger and Tada, 1976), a pentameric integral protein (Mr ≈ 6 kDa) of sarcoplasmic reticulum (SR), is an endogenous inhibitor of the SR Ca2+-ATPase. PLN plays a crucial role in excitation-contraction coupling in the heart. Phosphorylation of PLN catalyzed by cyclic AMP-dependent protein kinase activates the SR Ca2+-ATPase in myocardial cells (Tada and Katz, 1982) resulting in the rapid removal of Ca2+ ions from the myoplasm to lower the intracellular Ca2+ concentration ([Ca2+]i) during each excitation-contraction cycle. The cDNA of PLN has been cloned from the canine heart (Fujii et al., 1987), and subsequently from both cardiac and slow-twitch skeletal muscle of the rabbit (Fujii et al., 1988).

Keywords

Sarcoplasmic Reticulum Myosin Light Chain Kinase Aortic Smooth Muscle Cell Cardiac Muscle Cell Canine Heart 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Kwang S. Hwang
    • 1
  • Bernardo Nadal-Ginard
    • 1
  1. 1.Laboratory of Molecular and Cellular Cardiology and Howard Hughes Medical InstituteChildren’s Hospital Harvard Medical SchoolBostonUSA

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