Regulation of Smooth Muscle Myosin Light Chain Kinase by Calmodulin
Whereas Ca2+ serves as the primary intracellular messenger for regulation of contraction in all types of muscle, the underlying molecular mechanisms are different. Striated muscles utilize troponin C as the Ca2+ receptor and contain an array of cell specific proteins involved in maintenance of Ca2+ homeostasis. Phosphorylation reactions are important in regulation of contraction but phosphorylation of myosin light chains does not play a major role in force generation (Sweeney and Stull, 1990). Smooth muscles utilize calmodulin as the Ca2+ receptor and phosphorylation of myosin light chains is the rate-limiting reaction in contractility (Kamm and Stull, 1985). Since myosin light chain kinase is the primary enzyme that catalyzes light chain phosphorylation, analysis of this complex activation process is crucial to the understanding of how smooth muscles move.
KeywordsMyosin Light Chain Myosin Light Chain Kinase Rabbit Skeletal Muscle Amino Acid Repeat Calmodulin Binding
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