Characteristics of the Voltage-Dependent Calcium Channel in Smooth Muscle: Patch-Clamp Studies

  • Kenji Kitamura
  • Noriyoshi Teramoto
  • Masahiro Oike
  • Zhiling Xiong
  • Shunichi Kajioka
  • Yoshihito Inoue
  • Bernd Nilius
  • Hirosi Kuriyama
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 304)


Visceral smooth muscle cells, including vascular smooth muscle cells, possess various types of Ca channels. The voltage-dependent Ca channel is commonly observed in many tissues and is thought to play an important role in the generation of action potentials. Neural transmitters, hormones, autacoids, peptides and other substances activate individual receptors and cause activation of the receptor-operated ion channels which are permeable to Na and Ca, and in some tissues, CI ion. Thus, receptor activation may induce an influx of Ca via activation of the receptor-operated channel and voltage-dependent Ca channel, and also induce release of Ca from the sarcoplasmic reticulum (SR) via synthesis of inositol 1,4,5-trisphosphate (IP3). In addition, the concentration gradient between extra- and intra-cellular Ca (2.5 mM and 100 nM, respectively) may promote the passive influx of Ca. However, analysis of this current has not yet been made in detail. In this chapter, we discuss mainly the features of the voltage-dependent Ca channel recorded from visceral smooth muscle cells using voltage- and patch-clamp procedures, and also compare their characteristics to those in cardiac muscle cells.


Smooth Muscle Cell Cardiac Muscle Cell Single Smooth Muscle Cell Smooth Muscle Cell Membrane Smooth Muscle Membrane 


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© Plenum Press, New York 1991

Authors and Affiliations

  • Kenji Kitamura
    • 1
  • Noriyoshi Teramoto
    • 1
  • Masahiro Oike
    • 1
  • Zhiling Xiong
    • 1
  • Shunichi Kajioka
    • 1
  • Yoshihito Inoue
    • 1
  • Bernd Nilius
    • 1
  • Hirosi Kuriyama
    • 1
  1. 1.Department of Pharmacology Faculty of MedicineKyushu UniversityFukuokaJapan

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