Calcium Homeostasis in Single Intact Smooth Muscle Cells
The force of contraction of smooth muscle cells, is a function of the Ca2+ ion activity of the myoplasm (Fay et al., 1974). There has therefore been a tremendous effort directed at understanding Ca2+ homeostatic mechanisms, with experiments ranging the spectrum from isolated genes to intact cells. The work of the scientists in this laboratory has concentrated on the latter, Ca2+ regulation in intact, single smooth muscle cells that have been enzymatically isolated from the stomach muscularis of the toad Bufo marinus (Fay et al., 1982). These experiments became possible only because of key technological developments of the last decade. In particular, it was the rational design of ion-sensitive fluorescent dyes that could be used ratiometrically to measure ion concentrations in living cells (Grynkiewicz et al, 1985) and the marriage of this technique with digital imaging microscopy (Fay et al, 1986), which enabled our experimental approach.
KeywordsSmooth Muscle Cell Myosin Light Chain Kinase Calmodulin Dependent Protein Kinase Stomach Smooth Muscle Calmodulin Binding Domain
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