Preparation and Characterization of Antisera and of Murine Monoclonal Antibodies to Human Glioma-Associated Antigen(s)

  • Masafumi Matsuda
  • Duncan K. Fischer
  • Raj K. Narayan
  • M. Zouhair Atassi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 303)


Human glioma-associated markers can be exploited for the development of new diagnostic strategies and treatment modalities for these malignancies. A goat antiserum was first raised against human anaplastic astrocytoma (AC or AA) and glioblastoma multiforme (GB or GBM) extracts. Extensive sequential absorptions with normal brain tissue, normal serum, and human serum albumin (HSA) gave an antibody fraction specific for glioma. Balb/c mice were subsequently immunized with these glioma extracts. B-cell hybridomas from these mice were then cloned and subcloned by limiting dilution, yielding six monoclonal antibodies (MAbs) that were entirely specific for tumor tissues, and did not react with normal human serum or with normal human brain, liver, kidney, spleen, or muscle. Moreover, the murine MAbs did not cross-react with certain other human tumors, including melanoma. The fully absorbed antiserum and the murine MAbs both identify a polypeptide pattern possibly related to human glial fibrillary acidic protein (GFAP) or other intermediate filament proteins on immunoblots. These immunological reagents could serve as powerful tools for the diagnosis and possibly therapy of these uniformly fatal tumors.


Human Serum Albumin Glial Fibrillary Acidic Protein Glioblastoma Multiforme Anaplastic Astrocytoma Normal Brain Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Masafumi Matsuda
    • 2
  • Duncan K. Fischer
    • 2
  • Raj K. Narayan
    • 2
  • M. Zouhair Atassi
    • 1
  1. 1.Department of BiochemistryBaylor College of MedicineHoustonUSA
  2. 2.Department of NeurosurgeryBaylor College of MedicineHoustonUSA

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