Autoimmune Recognition Profile of the Alpha Chain of Human Acetylcholine Receptor in Myasthenia Gravis

  • Tetsuo Ashizawa
  • Minako Oshima
  • Ke-He Ruan
  • M. Zouhair Atassi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 303)

Abstract

Myasthenia gravis (MG) is an autoimmune disease with skeletal muscle weakness and easy fatigability due to autoantibodies directed against acetylcholine receptor (AChR) molecules at the motor endplates. The AChR consists of 2 alpha (α) subunits and one each of beta, gamma and delta subunit in a circular arrangement. Studies have shown that major populations of the autoantibodies are directed against extracellular domains of the α subunit which bears binding sites for the ligands and α-toxin (reviewed in Ashizawa and Appel, 1985; Atassi et al, 1987).

Keywords

Acetylcholine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ashizawa, T., and Appel, S. H., 1985, Immunopathological events at the end-plate in myasthenia gravis, Springer Sem. Immunopathol., 8:117.Google Scholar
  2. Atassi, M.Z. and Smith, J.A., 1978, A proposal for the nomenclature of antigen sites in peptides and proteins, Immunochem., 15:609.CrossRefGoogle Scholar
  3. Atassi, M.Z., Mulac-Jericevic, B., Yokoi, T. and Manshouri, T., 1987, Localization of the functional sites on the α chain of acetylcholine receptor, Fed. Proc., 46:2538.PubMedGoogle Scholar
  4. Kazim, A.L. and Atassi, M.Z., 1980, A novel and comprehensive synthetic approach for the elucidation of protein antigenic structures. Determination of the full antigenic profile of the a-chain of human hemoglobin, Biochem. J., 191:613.Google Scholar
  5. Kazim, A.L. and Atassi, M.Z., 1982, Structurally inherent antigenic sites: Localization of the antigenic sites of the a-chain of human haemoglobin in three host species by a comprehensive synthetic approach, Biochem. J., 203:201.PubMedGoogle Scholar
  6. Mulac-Jericevic, B., Kurisaki, J., and Atassi, M. Z., 1987, Profile of the continuous antigenic regions on the extracellular part of the α chain of an acetylcholine receptor, Proc. Natl. Acad. Sci. USA, 84:3633.PubMedCrossRefGoogle Scholar
  7. Mulac-Jericevic, B., Manshouri, T., Yokoi, T., and Atassi, M. Z., 1988, The regions of a-neurotoxin binding on the extracellular part of the α-subunit of human acetylcholine receptor, J. Prot. Chem., 7:173.CrossRefGoogle Scholar
  8. Neitzel, H., 1986, A routine method for the establishment of permanent growing lymphoblastoid cell lines, Hum. Genet., 73:320.PubMedCrossRefGoogle Scholar
  9. Noda, M., Takahashi, H., Tanabe, T., Toyosato, M., Furutani, Y., Hirose, T., Asai, M., Inayama, S., Miyata, T., and Numa, S., 1982, Primary structure of α-subunit precursor of Torpedo californica acetylcholine receptor deduced from cDNA sequences, Nature, 299:793.PubMedCrossRefGoogle Scholar
  10. Noda, M., Furutani, Y., Takahashi, H., Toyosato, M., Tanabe, T., Shimizu, S., Kikyotani, S., Kayano, T., Hirose, T., Inayama, S., and Numa, S., 1983, Cloning and sequence analysis of calf cDNA and human genomic DNA encoding a-subunit precursor of muscle acetylcholine receptor, Nature, 305:818.PubMedCrossRefGoogle Scholar
  11. Oshima, M, Ashizawa, T., Pollack, M.S., and Atassi, M.Z., 1990, Autoimmune T cell recognition of human acetylcholine receptor: The sites of T cell recognition in myasthenia gravis on the extracellular part of the α subunit, Eur. J. Immunol.20:2563.PubMedCrossRefGoogle Scholar
  12. Pachner, A. R., Kantor, F. S., Mulac-Jericevic, B., and Atassi, M. Z., 1989, An immunodominant site of acetylcholine receptor in experimental myasthenia mapped with T lymphocyte clones and synthetic peptides, Immunol. Lett., 20:199.PubMedCrossRefGoogle Scholar
  13. Yoshioka, M., Bixler, G.S., and Atassi, M.Z., 1983, Preparation of T-lymphocyte lines and clones with specificities to preselected protein sites by in vitro passage with free synthetic peptides: demonstration with myoglobin sites, Mol. Immunol. 20:1133.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Tetsuo Ashizawa
    • 2
  • Minako Oshima
    • 1
  • Ke-He Ruan
    • 1
  • M. Zouhair Atassi
    • 1
  1. 1.Department of BiochemistryBaylor College of MedicineHoustonUSA
  2. 2.Department of NeurologyBaylor College of MedicineHoustonUSA

Personalised recommendations