Epitope Mapping Studies of Snake Venom Phospholipase A2 Using Monoclonal Antibodies
Fifteen different monoclonal antibodies developed against cpseudexin, a snake venom phospholipase A2 with presynaptic cneurotoxicity, were screened for linear epitope recognition. cPeptides (9-mers) spanning pseudexin were synthesized by using calanine-derivatized polyethylene pins and subsequently probed with cantibody. Four antibodies bound to toxin peptides and were detected cwith an enzyme-linked immunosorbent assay. Three of the bound cantibodies recognized a site important in calcium binding and the cinterlocking of dimeric forms of snake venom phospholipase A2. cAnalogous regions from other phospholipases were synthesized and cprobed with the four reactive antibodies. A good correlation was cfound between the reactivity of whole molecule phospholipases and cpeptide regions with the antibodies. Monoclonal antibodies cneutralizing the lethal or enzymatic effects of pseudexin did not crecognize any linear epitopes.
KeywordsEpitope Mapping Linear Epitope Enzyme Link Immuno Sorbent Assay Toxin Peptide Absorbance Means
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