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Antigenic Mapping of Light Chains and T Cell Receptor β Chains

  • J. J. Marchalonis
  • F. Dedeoglu
  • V. S. Hohman
  • K. McGee
  • S. F. Schluter
  • A. B. Edmundson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 303)

Abstract

Rearranging immunoglobulins form a closely homologous family of recognition and defense proteins specified by variable (V), joining (J) and constant (C) gene segments that encode peptide segments showing >50%, >70% and approximately 40% identity in phylogenetic comparisons from shark to man (1,2). This family also includes the rearranging T cell receptors that are formed from the same type of elements and show comparable degrees of identity to the corresponding segments of immunoglobulins (1,3,4). There has been considerable interest in antigenic determinants associated with the combining sites of antibodies (idiotypes) that are determined by the complementarity determining regions (CDR). However, overall antigenic mapping of immunoglobulin molecules has not been performed as it has for other proteins including lysozyme (5), myoglobulin (6) and albumin (7).

Keywords

Light Chain Antigenic Determinant Rabbit Antiserum Normal Rabbit Serum Complementarity Determine Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Marchalonis, J.J. and Schluter, S.F. Evolution of variable and constant domains and joining segments of rearranging immunoglobulins. The FASEB J. 3:2469–2479, (1989).Google Scholar
  2. 2.
    Schluter, S.F., Edmundson, A.B. and Marchalonis, J.J. Evolution of immunoglobulin light chains: complementary DNA clones specifying sandbar shark constant regions. Proc. Natl.Acad.Sci.USA 86:9961–9965, (1990).CrossRefGoogle Scholar
  3. 3.
    Toyonaga, B. and Mak, T.W. Genes of the T-cell antigen receptor in normal and malignant T cells. Ann. Rev. Immunol. 5:585, 1987.CrossRefGoogle Scholar
  4. 4.
    Kronenberg, M., Siu, G., Hood, L.E. and Shastri, N. The molecular genetics of the T-cell antigen receptor and T-cell antigen recognition. Ann. Rev. Immunol. 4:529 (1986).CrossRefGoogle Scholar
  5. 5.
    Atassi, M.Z., Determination of the entire antigenic structure of lysozyme by surface-simulation synthesis: a novel concept in molecular recognition. CRC Crit.Rev. Biochem. 6:371, (1979).PubMedCrossRefGoogle Scholar
  6. 6.
    Atassi, M.Z. Antigenic structure of myoglobin. THe complete immunochemical anatomy of a protein and conclusion relating to antigenic structures of proteins. Immunochem. 14:423 (1975).CrossRefGoogle Scholar
  7. 7.
    Sakata, S., and Atassi, M.Z. Immunochemistry of serum albumin. X. Five major antigenic sites of human serum albumin are extrapolated from bovine serum albumin and confirmed by synthetic peptides. Mol. Immunol. 17:139 (1980).PubMedCrossRefGoogle Scholar
  8. 8.
    Edmundson, A.B., Ely, K.R., Abola, E.E., Schiffer, M. and Panagotopoulos, N. Rotational allomerism and divergent evolution of domains in immunoglobulin light chains. Biochem. 14:3953 (1975).CrossRefGoogle Scholar
  9. 9.
    Schluter, S.F., Beischel, C.J., Martin, S.A. and Marchalonis, J.J. Sequence analysis of homogeneous peptides of shark immunoglobulin light chains by tandem mass spectrometry: Correlation with gene sequence and homologies among variable and constant region peptides of sharks and mammals. Mol. Immunol. 26:611–624, 1990.Google Scholar
  10. 10.
    Marchalonis, J.J., Hohman, V.S. and Schluter, S.F. The usefulness of heterologous antibody and molecular probes in studies of the evolution of antibodies, in: “Evolution of the Immune System”, Charlemagne, J. and Tournefier, H, eds., Telford Press, Caldwell, N.J., in press.Google Scholar
  11. 11.
    Yanagi, Y., Yoshikai, Y., Leggett, K., Clark, S.P., Aleksander, I., Mak, T.W. A human T cell specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains. Nature 308:145–149 (1984).PubMedCrossRefGoogle Scholar
  12. 12.
    Marchalonis, J.J., Warr, G.W., Wang, A.-C, Burns, W.H. and Burton, R.C. A Fab-related surface component of normal and neoplastic human and marmoset T cells: Demonstration, functional analysis and partial characterization. Mol. Immunol. 17:877–891 (1980).PubMedCrossRefGoogle Scholar
  13. 13.
    Schluter, S.F. and Marchalonis, J.J. Antibodies to synthetic joining segment peptide of the T-cell receptor β-chain: serological cross-reaction between products of T-cell receptor genes, antigen binding T-cell receptors and immunoglobulins. Proc. Natl. Acad. Sci. USA 83:1872–1876, (1986).PubMedCrossRefGoogle Scholar
  14. 14.
    Marchalonis, J.J., Schluter, S.F., Hubbard, R.A., McCabe, C., and Allen, R.C. Immunoglobulin epitopes defined by synthetic peptides corresponding to joining-region sequence: Conservation of determinants and dependence upon the presence of an arginyl or lysyl residue for cross-reaction between light chains and T cell receptor chains. Mol. Immunol. 25:771–784 (1988).PubMedCrossRefGoogle Scholar
  15. 15.
    Schluter, S.F., Rosenshein, I.L., Hubbard, R.A. and Marchalonis, J.J. Conservation among vertebrate immunoglobulin chains detected by antibodies to a synthetic joining segment peptide. Biochem. Biophys. Res.Comm. 145:699–705 (1987)PubMedCrossRefGoogle Scholar
  16. 16.
    Beale, D., and Coadwell, J. Unusual features of the T-cell receptor C domains are revealed by structural comparisons with other members of the immunoglobulin superfamily. Comp. Biochem. Physiol. 85B:205 (1986).Google Scholar
  17. 17.
    Shankey, T.V., Schluter, S.F. and Marchalonis, J.J. Flow cytometric analysis of human lymphocytes using affinity purified antibody to T cell receptors synthetic J region. Cell Immunol 118:526–531 (1989).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • J. J. Marchalonis
    • 1
  • F. Dedeoglu
    • 1
  • V. S. Hohman
    • 1
  • K. McGee
    • 1
  • S. F. Schluter
    • 1
  • A. B. Edmundson
    • 2
  1. 1.Microbiology and ImmunologyUniversity of ArizonaTucsonUSA
  2. 2.Harrington Cancer Ctr.AmarilloUSA

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