Antigenic Mapping of Light Chains and T Cell Receptor β Chains
Rearranging immunoglobulins form a closely homologous family of recognition and defense proteins specified by variable (V), joining (J) and constant (C) gene segments that encode peptide segments showing >50%, >70% and approximately 40% identity in phylogenetic comparisons from shark to man (1,2). This family also includes the rearranging T cell receptors that are formed from the same type of elements and show comparable degrees of identity to the corresponding segments of immunoglobulins (1,3,4). There has been considerable interest in antigenic determinants associated with the combining sites of antibodies (idiotypes) that are determined by the complementarity determining regions (CDR). However, overall antigenic mapping of immunoglobulin molecules has not been performed as it has for other proteins including lysozyme (5), myoglobulin (6) and albumin (7).
KeywordsLight Chain Antigenic Determinant Rabbit Antiserum Normal Rabbit Serum Complementarity Determine Region
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