Abstract
B lymphocytes are produced in fetal liver and bone marrow from multipotent precursor cells. The possibility of manipulating the expansion and differentiation of those precursors would allow the identification of the different steps involved in this process, and lead to the identification of growth factors required for the transition along the B-cell differentiation pathway. Major efforts have, therefore, been concentrated on the definition of such culture systems. Immature B-cells which lack detectable amounts of immunoglobulin on the surface and that are unresponsive to mitogens require particular culture conditions. Until recently, the only way to support growth and differentiation of B-cell precursors “in vitro” was to use adherent stromal cells from lymphoid organs. We will, therefore, concentrate our discussion on the role of stromal cells and soluble factors they produce in the generation of B lymphocytes from committed B-cell precursors.
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Collins, L. S. and Dorshkind, K., 1987. A stromal cell line from myeloid long-term bone marrow cultures can support myelopoiesis and B lymphopoiesis. J. Immunol. 138:1082.
Cumano, A., Dorshkind, K., Gillis, S. and Paige, C. J., 1990. The influence of S17 stromal cells and interleukin 7 on B cell development. Eur. J Immunol. 20:2183.
Hayashi, S.-L, Kunisada, T., Ogawa, M., Sudo, T., Kodama, H., Suda, T., Nishikawa, S. and Nishikawa, S.-L, 1990. Stepwise progression of B lineage differentiation supported by interleukin 7 and other stromal cell molecules. J. Exp. Med. 171:1683.
Huber, B. and Melchers, F., 1979. Frequencies of mitogen-reactive B cells in the mouse. Lipopolyssacharide-, lipoprotein-and Nocardia mitogen-reactive B cells in CBA/N mice. Eur. J. Immunol. 9:827.
Kincade, P. W., 1977. Defective colony formation by lymphocytes from CBA/N and C3H/HeJ mice. J Exp. Med. 145:249.
Kincade, P. W., 1988. Experimental models for understanding B lymphocyte formation. Adv. Immunol. 41:181.
Mosier, D. E., Scher, I. and Paul, W. E., 1976. In vitro responses of CBA/N mice: spleen cells of mice with an X-linked defect that precludes immune responses to several thymus-independent antigens can respond to TNP-lipopolyssacharide. J. Immunol. 117:1363.
Namen, A. E., Lupton, S., Hjerrild, K., Wignall, J., Mochizuki, D. Y., Schmierer, A., Mosley, B., March, C. J., Urdal, D., Gillis, S. and Goodwin, R. G. 1988. Stimulation of B-cell progenitors by cloned murine interleukin 7. Nature 333:571.
Paige, C. J. and Skarvall, H. J., 1982. Plaque formation by B cell colonies. J Immunol. Methods. 52:51.
Paige, C. J., 1983. Surface immunoglobulin-negative B-cell precursors detected by formation of antibody-secreting colonies in agar. Nature 302:711.
Pietrangeli, C. E., Hayshy, S.-I. and Kincade, P. W., 1988. Stromal cell lines which support lymphocyte growtth: characterization, sensitivity to radiation and responsivness to growth factors. Eur. J. Immunol. 18:863.
Sauter, H. and Paige, C. J., 1987. Detection of normal B-cell precursors that give rise to colonies producing both k and 1 immunoglobulin chains. Proc. Natl. Acad. Sci. USA 84:4989.
Scher, I. 1982. The CBA/N mouse strain: An experimental model illustrating the influence of the X-chromossome on immunity. Adv. Immulogy 33:1.
Welch, P. A., Burrows, P. D., Namen, A., Gillis, S. and Cooper, M. D., 1990. Bone marrow stromal cells and interleukin-7 induce coordinate expression of the BP-1/6C3 antigen and pre-B cell growth. International Immunology 2:697.
Wu, G. E. and Paige, C. J., 1988. VH gene family utilization is regulated by a locus outside of the VH region. EMBO J 5:3475.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Plenum Press, New York
About this chapter
Cite this chapter
Cumano, A., Narendran, A., Paige, C.J. (1991). Factors Produced by Stromal Cells Involved in B-Cell Development. In: Atassi, M.Z. (eds) Immunobiology of Proteins and Peptides VI. Advances in Experimental Medicine and Biology, vol 303. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-6000-1_20
Download citation
DOI: https://doi.org/10.1007/978-1-4684-6000-1_20
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-6002-5
Online ISBN: 978-1-4684-6000-1
eBook Packages: Springer Book Archive