Factors Produced by Stromal Cells Involved in B-Cell Development
B lymphocytes are produced in fetal liver and bone marrow from multipotent precursor cells. The possibility of manipulating the expansion and differentiation of those precursors would allow the identification of the different steps involved in this process, and lead to the identification of growth factors required for the transition along the B-cell differentiation pathway. Major efforts have, therefore, been concentrated on the definition of such culture systems. Immature B-cells which lack detectable amounts of immunoglobulin on the surface and that are unresponsive to mitogens require particular culture conditions. Until recently, the only way to support growth and differentiation of B-cell precursors “in vitro” was to use adherent stromal cells from lymphoid organs. We will, therefore, concentrate our discussion on the role of stromal cells and soluble factors they produce in the generation of B lymphocytes from committed B-cell precursors.
KeywordsStromal Cell Fetal Liver Stromal Cell Line Fetal Liver Cell Colony Assay
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