Factors Produced by Stromal Cells Involved in B-Cell Development

  • A. Cumano
  • A. Narendran
  • C. J. Paige
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 303)


B lymphocytes are produced in fetal liver and bone marrow from multipotent precursor cells. The possibility of manipulating the expansion and differentiation of those precursors would allow the identification of the different steps involved in this process, and lead to the identification of growth factors required for the transition along the B-cell differentiation pathway. Major efforts have, therefore, been concentrated on the definition of such culture systems. Immature B-cells which lack detectable amounts of immunoglobulin on the surface and that are unresponsive to mitogens require particular culture conditions. Until recently, the only way to support growth and differentiation of B-cell precursors “in vitro” was to use adherent stromal cells from lymphoid organs. We will, therefore, concentrate our discussion on the role of stromal cells and soluble factors they produce in the generation of B lymphocytes from committed B-cell precursors.


Stromal Cell Fetal Liver Stromal Cell Line Fetal Liver Cell Colony Assay 
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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • A. Cumano
    • 1
  • A. Narendran
    • 1
  • C. J. Paige
    • 1
  1. 1.The Ontario Cancer InstituteTorontoCanada

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