Clonal Analysis of Neoplastic Transformation in Cultured Diploid Rat Liver Epithelial Cells

  • J. W. Grisham
  • M.-S. Tsao
  • L. W. Lee
  • G. J. Smith
Part of the Basic Life Sciences book series (BLSC, volume 57)


The organizers of this symposium posed specific queries to the participants. In this session we were asked to address this question: “At which point in time does genetic instability occur in the natural history of cancer?” We were also asked to consider “mechanistic similarities between progression and promotion” and “to suggest research needed to elucidate these issues.” Although complete answers to these questions are not yet available, we will examine these general issues in the context of studies being performed in our laboratory to clonally analyze the process of transformation in vitro in cultured diploid rat liver epithelial cells1–10. Thus far our studies have concentrated on identifying the essential tumorigenic phenotype/ genotype in cells of this model system by analyzing the clonal cosegregation of phenotypic/genotypic properties with tumorigenicity. Clonal analysis also provides a powerful strategy for identifying a lineal linkage between various nontumorigenic and the tumorigenic variants. Although we have not yet accomplished complete or detailed lineage tracing, we are working toward this goal after establishing some of the critical features of the tumorigenic phenotype/genotype. Our early results allow the outline of a possible lineage to be inferred.


Clonal Line Clonal Analysis Phenotypic Property Tumorigenic Cell Stem Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


alp and TGF-alpha

Transforming growth factor-alpha




epidermal growth factor


gamma glutamyl transpeptidase


glucose 6-phosphate dehydrogenase: GLC, capacity to form colonies in medium containing low levels of calcium


lactate dehydrogenase




polyadenlyated messenger ribonucleic acid


gene coding for genomic sequences that are homologous with some of those of the avian MC29 (myelocytomatosis) virus


pyruvate kinase


capacity to form colonies in soft agar


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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • J. W. Grisham
    • 1
  • M.-S. Tsao
    • 2
  • L. W. Lee
    • 1
  • G. J. Smith
    • 1
  1. 1.Department of PathologyCB 7525 University of North Carolina at Chapel HillChapel HillUSA
  2. 2.Department of PathologyMontreal General Hospital and McGill UniversityMontrealCanada

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