Tumor Progression in Transgenic Mice Containing the Bovine Papillomavirus Genome

  • Peter M. Howley
Part of the Basic Life Sciences book series (BLSC, volume 57)


Transgenic mice are an interesting system to study a variety of biological phenomena. It has been a particularly interesting way of studying oncogenesis. It has provided a tractable system to study and to dissect the multiple steps involved in carcinogenesis, essentially by fixing one of these steps1. In transgenic mice, DNA is introduced into the germ line and that gene is stably inherited to the offspring of the mice. Transgenic mice have been developed using a 1.69 tandemly reiterated copy of the bovine papillomavirus type 1 (BPV-1) genome2. BPV-1 encodes two different viral oncogenes, and in the transgenic line of mice that has been developed, these two viral oncogenes are under the control of the transcriptional regulatory sequences of the virus to ensure proper tissue specific expression of the viral oncogenes.


Transgenic Mouse Human Papilloma Virus Dermal Fibroblast Viral Oncogene Viral Gene Expression 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    D. Hanahan, Oncogenesis in transgenic mice, in: “Oncogenes and Growth Control,” T. Graf and P. Kahn eds., Springer-Yerlag KG, Berlin (1986), pp. 349–363.CrossRefGoogle Scholar
  2. 2.
    M. Lacey, S. Alpert, and D. Hanahan, The bovine papillomavirus virus genome elicits skin tumors in transgenic mice, Nature 322:609–612 (1986).PubMedCrossRefGoogle Scholar
  3. 3.
    C. C. Baker and P. M. Howley, Differential promoter utilization by the bovine papillomavirus in transformed cells and in productively infected wart tissues, EMBO J. 6:1027–1035 (1987).PubMedGoogle Scholar
  4. 4.
    P. F. Lambert, C. C. Baker, and P. M. Howley, The genetics of bovine papillomavirus type 1, Ann. Rev. Genetics 22:235–258 (1988).CrossRefGoogle Scholar
  5. 5.
    R. Schlegel, M. Wade-Glass, M. S. Rabson, and Y. C. Yang, The E5 transforming gene of bovine papillomavirus encodes a small hydrophobic polypeptide. Science 233:464–467, (1986).PubMedCrossRefGoogle Scholar
  6. 6.
    E. J. Androphy, J. T. Schiller, and D. R. Lowy, Identification of the protein encoded by the E6 transforming gene of bovine papillomavirus, Science 230:442–445 (1985).PubMedCrossRefGoogle Scholar
  7. 7.
    P. M. Howley and R. Schlegel, Papillomavirus transformation, in: “The Papovaviridae 2: the papillomaviruses,” N. P. Salzman and P. M. Howley eds., Plenum Publishing Corp., New York (1987), pp. 144–166.Google Scholar
  8. 8.
    M. Sippola-Thiele, D. Hanahan, and P. M. Howley, Cell-heritable stages of tumor progression in transgenic mice harboring the bovine papillomavirus type 1 genome, Mol. Cell. Biol. 9:925–934 (1989).PubMedGoogle Scholar
  9. 9.
    V. Lindgren, M. Sippola-Thiele, J. Skowronski, E. Wetzel, P. M. Howley, and D. Hanahan, Specific chromosomal abnormalities characterize fibrosarcomas of bovine papillomavirus-1 transgenic mice, Proc. Natl. Acad. Sci. USA, in press (1989).Google Scholar
  10. 10.
    J. C. Stone, J. L. Crosby, C. A. Kozak, A. R. Shievella, R. Bernards, and J. H. Nadeau, The murine retinoblastoma homologue maps to chromosome 14 near Es-10, Genomics, in press (1989).Google Scholar
  11. 11.
    P. Frost, R. S. Kerbel, B. Hunt, S. Man, and S. Pathak, Selection of metastatic variance with identifiable karyotypic changes from a nonmetastatic murine tumor after treatment with 2′-Deoxy-5-azacytidine or hydrourea: implications for the mechanism of tumor progression, Cancer Res. 47:2690–2695 (1987).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Peter M. Howley
    • 1
  1. 1.Laboratory of Tumor Virus BiologyNational Cancer InstituteBethesdaUSA

Personalised recommendations