High-Fluence Rate Monochromatic Light Sources, Computerized Analysis of Cell Movements, and Microbeam Irradiation of a Moving Cell: Current Experimental Methodology at the Okazaki Large Spectrograph

  • Masakatsu Watanabe
Part of the NATO ASI Series book series (NSSA, volume 211)


Previous biological spectrographs (Parker et al., 1946; Monk and Ehret, 1956; Jacques et al., 1964; Balegh and Biddulph, 1970; Watanabe and Furuya, 1974) which enabled simultaneous irradiation of many samples with different wavelengths have been very useful tools for action spectroscopy, especially with whole plant systems (Borthwick et al, 1952; Shen-Miller et al, 1969; Wada and Furuya, 1974; Inoue and Furuya, 1975).


Action Spectrum Phototactic Response Microbeam Irradiation Posterior Flagellum Action Spectroscopy 
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  1. Arikawa, K., and Aoki, K., 1982, Response characteristics and occurrence of extraocular photoreceptors on lepidopteran genitalia, J. Comp. Physiol., 148:483.CrossRefGoogle Scholar
  2. Balegh, S. E., and Biddulph, O., 1970, The photosynthetic action spectrum in the bean plant, Plant Physiol., 46:1.PubMedCrossRefGoogle Scholar
  3. Baskin, T. I., and Iino, M., 1987, An action spectrum in the blue and ultraviolet for phototropism in alfalfa, Photochem. Photobiol., 46:127.CrossRefGoogle Scholar
  4. Borthwick, H. A., Hendricks, S. B., Parker, M. W., Toole, E. H., and Toole, V. K., 1952, A reversible photoreaction controlling seed germination, Proc. Natl. Acad. ScL U. S. A., 38:662.CrossRefGoogle Scholar
  5. Boscov, J. S., and Feinleib, M. E., 1970, Phototactic response of Chlamydomonas to flashes of light. II. Response of individual cells, Photochem. Photobiol., 30:499.CrossRefGoogle Scholar
  6. Choi, K.-S., Watanabe, M., and Furuya, M., 1989, Effects of long-term storage on phytochrome-mediated germination in lettuce seeds, Bot. Mag. Tofcyo, 102:181.CrossRefGoogle Scholar
  7. Davenport, D., Culler, G. J., Greaves, J. O. B., Forward, R. B., and Hand, W. G., 1970, The investigation of the behavior of microorganisms by computerized television, IEEE Trans. Biomed. Engineer., 17:230.CrossRefGoogle Scholar
  8. Durand, R., and Furuya, M., 1985, Action spectra for stimulatory and inhibitory effects of UV and blue light on fruitbody formation in Coprinus congregatus, Plant Cell Physiol., 26:1175.Google Scholar
  9. Eker, A. P. M., 1978, Some properties of a DNA photoreactivating enzyme from Streptomyces griseus, in: “MDNA repair mechanisms,” P. C. Hanawalt, E. C. Friedberg, and C. F. Fox, eds., Academic Press, New York, p. 129.Google Scholar
  10. Eker, A. P. M., Dekker, R. H., and Berends, W., 1981, Photoreactivation enzyme from Streptomyces griseus. IV. On the nature of the chromophoric cofactor in Streptomyces griseus photoreactivating enzyme, Photochem. Photobiol., 33:65.PubMedCrossRefGoogle Scholar
  11. Feinlieb, M. E. H., and Curry, G. M., 1967, Methods for measuring phototaxis of cell populations and individual cells, Physiol. Plant., 20:1083.CrossRefGoogle Scholar
  12. Furuya, M., Wada, M., and Kadota, A., 1980, Regulation of cell growth and cell cycle by blue light in Adiantum gametophytes, in: “Blue Light Syndrome,” H. Senger, ed., Springer-Verlag, Berlin, p. 119.CrossRefGoogle Scholar
  13. Galland, P., and Senger, H., 1988a, The role of flavins as photoreceptors, J. Photochem. Photobiol. B: Biol., 1:277.CrossRefGoogle Scholar
  14. Galland, P., and Senger, H., 1988b, The role of pterins in the photoreception and metabolism of plants, Photochem. Photobiol., 48:811.CrossRefGoogle Scholar
  15. Häder, D.-P., and Lebert, M., 1985, Real time computer-controlled tracking of motile microorganisms, Photochem. Photobiol., 42:509.PubMedCrossRefGoogle Scholar
  16. Hand, W. G., and Davenport, D., 1970, The experimental analysis of phototaxis and photokinesis in flagellates, in: “Photobiology of Microorganisms,” P. Halldal, ed., Wiley, London, p. 253.Google Scholar
  17. Haupt, W., Mortel, G., and Winkelnkemper, I., 1969, Demonstration of different dichroic orientation of phytochrome Pr and Pfr, Planta, 88:183.CrossRefGoogle Scholar
  18. Iino, M., 1988, Pulse-induced phototropisms in oat and maize coleoptiles, Plant Physiol., 88:823.PubMedCrossRefGoogle Scholar
  19. Inoue, Y., and Furuya, M., 1975, Perithecial formation in Gerasinospora reticulispora. TV: Action spectra for the photoinduction, Plant Physiol., 55:1098.PubMedCrossRefGoogle Scholar
  20. Inoue, Y., and Watanabe, M., 1984, Perithecial formation in Gerasinospora reticulispora. VII: Action spectra in UV region for the photoinduction and the photoinhibition of photoinductive effect brought by blue light, Plant Cell Physiol., 25:107.Google Scholar
  21. Inoue, Y., 1984, Re-examination of action spectroscopy in blue/near-UV light effects, in: “Blue Light Effects in Biological Systems,” H. Senger, ed., Springer-Verlag, Berlin, p. 110.CrossRefGoogle Scholar
  22. Jacques, R., Chabbal, R., Couard, P., and Jacquinot, P., 1964, Mise au point d’un illuminateur spectral a usage biologique, C. R. Acad. Sci. Paris, 259:1581.Google Scholar
  23. Jagger, J., Takebe, H., and Snow, J. M., 1970, Photoreactivation of killing in Streptomyces: Action spectra and kinetic studies, Photochem. Photobiol., 12:185.PubMedCrossRefGoogle Scholar
  24. Kadota, A., Wayne, R., Kubota, M., and Watanabe, M., Localization of the photoreceptive site for the step-up photophobic response in a green alga, Dunaliella salina as analyzed by the partial irradiation of a cell, Photochem. Photobiol., in prep.Google Scholar
  25. Kawai, H., 1988, A flavin-like autofluorescent substance in the posterior flagellum of golden and brown algae, J. Phycol., 24:114.CrossRefGoogle Scholar
  26. Kawai, H., Müller, D. G., Fölster, E., and Häder, D.-P., 1990, Phototactic responses in the gametes of the brown alga, Ectocarpus siliculosus., Planta, 182:292.Google Scholar
  27. Kawai, H., Kubota, M., Kondo, T., and Watanabe, M., 1991, Action spectra for phototaxis in zoospores of the brown alga Pseudochorda gracilis, Protoplasma, in press.Google Scholar
  28. Kondo, T., Kubota, M., Aono, Y., and Watanabe, M., 1988, A computerized video system to automatically analyze movements of individual cells and its application to the study of circadian rhythms in phototaxis and motility in Chlamydomonas reinhardtii, Protoplasma, Suppl. 1:185.CrossRefGoogle Scholar
  29. Kondo, T., Johnson, C. H., and Hastings, J. W., 1991, Action spectrum for resetting the circadian phototaxis rhythm in the CW15 strain of Chlamydomonas. I. Cells in darkness, Plant Physiol., in press.Google Scholar
  30. Lend, F., and Ghetti, F., 1989, Photoreceptor pigments for photomovement of microorganisms: some spectroscopic and related studies, J. Photochem. Photobiol. B: Biol., 3:1.CrossRefGoogle Scholar
  31. Lumsden, P. J., Saji, H., and Furuya, M., 1987, Action spectra confirm two separate actions of phytochrome in the induction of flowering in Lemna paucicostata 441, Plant Cell Physiol., 28:1237.Google Scholar
  32. Monk, G. S., and Ehret, C. F., 1956, Design and performance of a biological spectrograph, Radiat. Res., 5:88.PubMedCrossRefGoogle Scholar
  33. Müller, D. G., Maier, I., and Müller, H., 1987, Flagellum autofluorescence and photoaccumulation in hete-rokont algae, Photochem. Photobiol., 46:1003.CrossRefGoogle Scholar
  34. Nultsch, W., and Häder, D.-P., 1988, Photomovement in motile microorganisms. II, Photochem. Photobiol., 47:837.PubMedCrossRefGoogle Scholar
  35. Ogura, T., Yoshikawa, S., and Kitagawa, T., 1985, Resonance Raman spectra of catalytic intermediates of cytochrome c oxydase detected with a mixed flow transient apparatus, Biochem. Biophys. Acta, 832:220.PubMedCrossRefGoogle Scholar
  36. Ogura, T., Takahashi, S., Shinzawa-Ito, K., Yoshikawa, S., and Kitagawa, T., 1990, Observation of the Fe4+ = O stretching Raman band for cytochrome oxidase compound B at ambient temperature, J. Biol. Chem., 265:14721.PubMedGoogle Scholar
  37. Ohki, K., Watanabe, M., and Fujita, Y., 1982, Action of near UV and blue light on the photocontrol of phycobiliprotein formation; a complementary chromatic adaptation, Plant Cell Physiol., 23:651.Google Scholar
  38. Parker, M. W., Hendricks, S. B., Borthwick, H. A., and Scully, N. J., 1946, Action spectrum for the photoperiodic control of floral initiation of short-day plants, Bot. Gaz., 108:1.CrossRefGoogle Scholar
  39. Saji, H., Furuya, M., and Takimoto, A., 1982, Spectral dependence of night-break effect on photoperiodic floral induction in Lemna paucicostata 441, Plant Cell Physiol., 23:623.Google Scholar
  40. Saji, H., Vince-Prue, D., and Furuya, M., 1983, Studies on the photoreceptors for the promotion and inhibition of flowering in dark-grown seedlings of Pharbitis nil Choisy, Plant Cell Physiol., 24:1183.Google Scholar
  41. Schmid, R., 1984, Blue light effects on morphogenesis and metabolism in Acetabularia, in: “Blue Light Effects in Biological Systems,” H. Senger, ed., Springer-Verlag, Berlin, p. 419.CrossRefGoogle Scholar
  42. Shen-Miller, J., Cooper, P., and Gordon, S. A., 1969, Phototropism and photoinhibition of basipolar transport of auxin in oat coleoptiles, Plant Physiol., 44:491.PubMedCrossRefGoogle Scholar
  43. Sugai, M., Tomizawa, K., Watanabe, M., and Furuya, M., 1984, Action spectrum between 250 and 800 nanometers for the photoinduced inhibition of spore germination in Pteris vittata, Plant Cell Physiol., 25:205.Google Scholar
  44. Sugai, M., and Furuya, M., 1985, Action spectrum in ultraviolet and blue light region for the inhibition of red-light-induced spore germination in Adiantum capillus-veneris L., Plant Cell Physiol., 26:953.Google Scholar
  45. Takahashi, T., and Kobatake, Y., 1982, Computer-linked automated method for measurement of the reversal frequency in phototaxis ofHalobacterium halobium, Cell Struct Funct., 7:183.CrossRefGoogle Scholar
  46. Ueda, T., Mori, Y., Nakagaki, T., and Kobatake, Y., 1988, Action spectra for superoxide generation and UV and visible light photoavoidance in plasmodia of Physarum polycephalum, Photochem. Photobiol., 48:705.CrossRefGoogle Scholar
  47. Wada, M., and Furuya, M., 1974, Action spectrum for the timing of photoinduced cell division in Adiantum gametophytes, Physiol. Plant., 32:377.CrossRefGoogle Scholar
  48. Wada, M., and Furuya, M., 1978, Effects of narrow-beam irradiations with blue and far-red light on the timing of cell division in Adiantum gametophytes, Planta, 126:85.CrossRefGoogle Scholar
  49. Watanabe, M., and Furuya, M., 1974, Action spectrum of phototaxis in a cryptomonad alga, Cryptomonas sp., Plant Cell Physiol., 15:413.Google Scholar
  50. Watanabe, M., and Furuya, M., 1982, Phototactic behavior of individual cells of Cryptomonas sp. in response to continuous and intermittent light stimuli, Photochem. Photobiol., 35:559.CrossRefGoogle Scholar
  51. Watanabe, M., Furuya, M., Miyoshi, Y., Inoue, Y., Iwahashi, I., and Matsumoto, K., 1982, Design and performance of the Okazaki Large Spectrograph for photobiological research, Photochem. Photobiol, 36:491.CrossRefGoogle Scholar
  52. Watanabe, M., 1985, The Okazaki Large Spectrograph and its application to action spectroscopy, in: “Photobiology 1984,” J. W. Longworth, J. Jagger, and W. Shropshire Jr., eds., Praeger, New York, p. 37.Google Scholar
  53. Watanabe, M., 1988, The Okazaki Large Spectrograph and the extension, into the ultraviolet region, of the action spectra for the signaling effects of blue and near-ultraviolet light in plants and fungi, Photo-med. Photobiol, 10:83.Google Scholar
  54. Watanabe, M., Kubota, M., Kadota, A., Morita, K., and Matsubara, M., A tracking microscope for micro-beam irradiation of a moving organism, Photochem. Photobiol, in prep.Google Scholar
  55. Wayne, R., Kadota, A., Watanabe, M., and Furuya, M., 1991, Photomovement in Dunaliella salina: Fluence rate-response curves and action spectra, Planta, in press.Google Scholar
  56. Yoshida, K., Takimoto, A., and Sasaki, Y., 1989, Fluence-response relationship for photogene expression in etiolated pea seedlings, Photochem. Photobiol, 50:121.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Masakatsu Watanabe
    • 1
  1. 1.National Institute for Basic BiologyOkazaki National Research InstitutesOkazaki, AichiJapan

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