Advertisement

Diabetes Mellitus and Red Blood Cell Aging: A Structural and Functional Study

  • Laura Mazzanti
  • Rosa A. Rabini
  • Roberto Staffolani
  • Emanuela Faloia
  • Roberto De Pirro
  • Armanda Pugnaloni
  • Gian Paolo Littarru
  • Graziella Biagini
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 307)

Abstract

Physiological insulin concentrations modulate the allosteric properties of membrane-bound enzymes, such as rat erythrocyte acetylcholinesterase (AchE) and Na+/K+ ATPase (1). Modifications in both AchE and Na+/K+-ATPase enzymatic activities have been observed in human erythrocyte membranes obtained from patients affected by Insulin- dependent-Diabetes Mellitus (IDDM) (2,3).

Keywords

Erythrocyte Membrane Diabetic Subject AchE Activity Human Erythrocyte Membrane Intramembrane Particle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    R. N. Farias, Membrane cooperative enzymes as a tool for the investigation of membrane structure and related phenomena, Adv. Lipid Res. 17:251 (1980).PubMedGoogle Scholar
  2. 2.
    I. Testa, R. A. Rabini, P. Fumelli, E. Bertoli and L. Mazzanti, Abnormal membrane fluidity and acetylchelinesterase activityin erythrocytes from insulin-dependent diabetic patients, J. Clin. Endocrinol Metab. 67(6):1129 (1988).PubMedCrossRefGoogle Scholar
  3. 3.
    L. Mazzanti, R. A. Rabini, I. Testa and E. Bertoli, Modifications induced by diabetes on the physico-chemical and functional properties of erythrocyte membrane, Eur. J. Clin. Invest. 19:84 (1989).PubMedGoogle Scholar
  4. 4.
    M. R. Clark, Senescence of red blood cells: Progress and Problems, Physiol. Rev. 68:503 (1988).PubMedGoogle Scholar
  5. 5.
    E. Beutler, C. West and K. G. Blume, The removal of leukocytes and platelets from whole blood, J. Lab. Clin. Med. 88:328 (1976).PubMedGoogle Scholar
  6. 6.
    A. Camagna, L. Rosseti, R. De Pirro, M. Di Franco, R. Lauro, P. Samoggia, P. Caprari and G. Salvo, Characterization of differences in insulin receptors from young and old red blood cells, J. Endocrinol. Invest. 10:371 (1987).PubMedGoogle Scholar
  7. 7.
    G. W. Burton and K. U. Ingold, K. E. Thompson, An improved procedure for the isolation of ghost membranes from human red blood cells, Lipids 16:946 (1981).PubMedCrossRefGoogle Scholar
  8. 8.
    G. L. Ellman, D.K. Courtney, V. Jr. Andres and R. M. Featherstone, A. new and rapid colorimetric determination of acetylcholinesterase activity, Biochem. Pharmacol. 7:88 (1961).PubMedCrossRefGoogle Scholar
  9. 9.
    O. H. Lowry, M. Y. Rosenburg, A. L. Farr and R. T. Randal, Protein measurement with the Folin phenol reagent, J. Biol. Chem. 193:265 (1951).PubMedGoogle Scholar
  10. 10.
    T. Kitao and K. Hattori, Inhibition of erythrocyte ATPase activity by aclacynomycin and reverse effects of ascorbate on ATPase activity, Experentia 39:1362 (1983).CrossRefGoogle Scholar
  11. 11.
    C. Fiske and Y. Subbarow, The colorimetric determination of phosphorus, J. Biol. Chem. 193:375 (1925).Google Scholar
  12. 12.
    J. H. Willison and A. J. Rowe, Replica, shadowing and freeze-etching techniques, in Pratical Methods in Electron Microscopy, Audrey M. Glauert, ed., North-Holland, Amsterdam (1980).Google Scholar
  13. 13.
    S. W. Last, L. J. Tertoolen and J. G. Bluemink, Quantitative analysis of the numerical and lateral distribution of intermembrane particles in freeze-fractures of biological membranes, Eur. J. Cell. Biol. 23:273 (1981).Google Scholar
  14. 14.
    J. Roos, M. Robinson and R. L. Davidson, Cell fusion and intramembrane particle distribution in polyethylene glycol-resistant cells, J. Cell Biol. 96:909 (1983).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Laura Mazzanti
    • 1
  • Rosa A. Rabini
    • 1
  • Roberto Staffolani
    • 1
  • Emanuela Faloia
    • 2
  • Roberto De Pirro
    • 2
  • Armanda Pugnaloni
    • 3
  • Gian Paolo Littarru
    • 1
  • Graziella Biagini
    • 3
  1. 1.Institute of BiochemistryUniversity of AnconaAnconaItaly
  2. 2.Institute of EndocrinologyUniversity of AnconaAnconaItaly
  3. 3.Institute of AnatomyUniversity of AnconaAnconaItaly

Personalised recommendations