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Clinical Utility of Fractionating Erythrocytes into “Percoll” Density Gradients

  • Andrea Mosca
  • Renata Paleari
  • Annalisa Modenese
  • Silvano Rossini
  • Rosalia Parma
  • Cristina Rocco
  • Vincenzo Russo
  • Giancarlo Caramenti
  • Maria Laura Paderi
  • Renzo Galanello
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 307)

Abstract

Two rapid methods for fractionating the RBC into five or nine layers of increasing density are reported. These procedures have been used to monitor the decline of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) activity during the process of red cell aging in normal subjects and in ß-thal carriers, to study transfused patients with G6PD and pyruvate kinase (PK) deficiency and to test the effects of inositol hexaphosphate (IHP) encapsulation on RBC subpopulations.

Keywords

Pyruvate Kinase G6PD Deficiency Blood Unit G6PD Activity Mean Cell Volume 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Andrea Mosca
    • 1
  • Renata Paleari
    • 2
  • Annalisa Modenese
    • 1
  • Silvano Rossini
    • 2
  • Rosalia Parma
    • 2
  • Cristina Rocco
    • 2
  • Vincenzo Russo
    • 3
  • Giancarlo Caramenti
    • 4
  • Maria Laura Paderi
    • 5
  • Renzo Galanello
    • 5
  1. 1.Dipt. Sc. Tecnol. BiomedicheUniv. MilanoItaly
  2. 2.Ist. Sci. H S. RaffaeleItaly
  3. 3.Ist. Fisiol. Veter. Biochim.Univ. MilanoItaly
  4. 4.I.T.B.A., C.N.R.MilanoItaly
  5. 5.1st. Clin. Biol. Età Evol.Univ. CagliariItaly

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