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Role of Hemoglobin Denaturation and Band 3 Clustering in Initiating Red Cell Removal

  • Philip S. Low
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 307)

Abstract

While multiple mechanisms likely exist to assure that a defective erythrocyte does not escape removal by macrophages, we believe that the more heavily used clearance pathways will have certain characteristics in common. First, the pathway should involve a change in components already present in the circulating erythrocytes, since de novo protein synthesis will have terminated before the erythrocyte reaches maturity. Second, the changes initiating the removal sequence must eventually be manifested on the exofacial surface of the cell, since a macrophage has little means of detecting an intracellular biochemical lesion. And finally, the exofacial changes recognized by the macrophage must be inducible by a change in the biochemistry of the cytoplasm, since cells that develop intracellular defects early in their lifespans are also removed early (e.g., sickle cells, (1) ß-thalassemic cells, (2) cells with enzyme deficiencies, (3) cells treated with oxidants, (4) etc). That is, a linkage of some sort must exist between the functional state of components in the cytoplasm and markers at the cell surface recognized by macrophages. The hypothesis outlined below describes how hemoglobin, the most abundant protein in the cytoplasm, and band 3, the most prominent protein in the membrane cooperate to establish this linkage, transducing information regarding the biochemical integrity of the cell to the reticuloendothelial system which is responsible for aged/abnormal cell clearance.

Keywords

Sickle Cell Human Erythrocyte Complement Fixation Cluster Agent Globin Chain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Philip S. Low
    • 1
  1. 1.Department of ChemistryPurdue UniversityWest LafayetteUSA

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