Significance and Relevance of NAD Synthesis in Human Erythrocyte Life Span

  • Vanna Micheli
  • Silvia Sestini
  • Marina Rocchigiani
  • Monica Pescaglini
  • Carlo Ricci
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 307)


NAD and NADP are known to play a key role in the general metabolism of human erythrocytes, owing to their involvement in all oxidative-reductive reactions occuring both in the energetic catabolism and in the processes preventing the cells from oxidative damage.


Intact Cell Human Erythrocyte Pyridine Nucleotide Crude Lysate Discontinuous Density Gradient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    J. Preiss, P. Handler, Biosynthesis of DPN. I. Identification of intermediates, J. Biol. Chem. 233:759 (1957).Google Scholar
  2. 2.
    H. A. Simmonds, L. D. Fairbanks, G. S. Morris, D. R. Webster, E. H. Harley, Altered erythrocyte patterns are characteristic of inherited disorders of purine and pyrimidine metabolism, Clin. Chim. Acta 171:197 (1988).PubMedCrossRefGoogle Scholar
  3. 3.
    C. R. Zerez, K. R. Tanaka, Impaired NAD synthesis in Pyruvate-Kinase-deficient human erythrocytes: a mechanism for decreased total NAD content and a possible secondary cause of hemolysis, Blood 69:999 (1987).PubMedGoogle Scholar
  4. 4.
    J. Preiss, P. Handler, Synthesis of DPN from NA by human erythrocytes in vitro, J. Amer. Chem. Soc. 79:1514 (1957).CrossRefGoogle Scholar
  5. 5.
    V. Micheli, S. Sestini, C. Ricci, Purine and pyridine nucleotide production in human erythrocytes, Arch. Biochem. Biophys. 244:54 (1986).CrossRefGoogle Scholar
  6. 6.
    V. Micheli, H. A. Simmonds, S. Sestini, C. Ricci, Importance of nicotinamide as an NAD precursor in the human erythrocyte, Arch. Biochem. Biophys. 283:40 (1990).PubMedCrossRefGoogle Scholar
  7. 7.
    V. Micheli S. Sestini, M. Rocchigiani, M. Pescaglini, C. Ricci, Nucleotide synthesis in the human erythrocyte: correlation between purines and pyridines, Biomed. Biochim. Acta 46:268 (1987).Google Scholar
  8. 8.
    S. Sestini, P. Lusini, V. Micheli, M. L. Ceccuzzi, C. Ricci, Evidence for NMN adenylyltransferase in human erythrocytes. Preliminary observations, Ital. J. Biochem. 37:63A (1988).Google Scholar
  9. 9.
    M. Rocchigiani, H. A. Simmonds, M. Bari, C. Ricci, NAmPRT assay in human red blood cells, Atti 35° Congresso Nazionale S.I.B., 1990.Google Scholar
  10. 10.
    M. Pescaglini, M. Rocchigiani, S. Sestini, V. Micheli, C. Ricci, Sintesi di NAD da Nicotinamide nei globuli rossi umani, Atti Accad. Fisiocritici Siena XV-Tomo VII:255 (1989).Google Scholar
  11. 11.
    V. Micheli, C. Ricci, A. Taddeo, R. Gili, Centrifugal fractionation of human erythrocytes according to age: comparison between Ficoll and Percoll density gradients, Quad. Sclavo Diagn. 22:236 (1985).Google Scholar
  12. 12.
    M. Rocchigiani, M. Pescaglini, S. Sestini, V. Micheli, C. Ricci, Nicotinic acid and Nicotinamide uptake by human erythrocytes, Ital. J. Biochem.38:159 (1989).Google Scholar
  13. 13.
    M. Rocchigiani, M. Bari, V. Micheli, M. Pescaglini, S. Sestini, Pyridine and purine nucleotide metabolism in human erythrocytes: HPLC determination of the intermediates, Ann. Nutr. Metab. 33:225 (1989).Google Scholar
  14. 14.
    C. Ricci, S. Sestini, V. Micheli, Role of NMN adenylyltransferase in the regulation of NAD metabolism of human erythrocytes, “Macromolecules in the functioning cell”, Proceedings of the 6th Soviet-Italian Symposium (Kiev, 1988), in press.Google Scholar
  15. 15.
    E. Beutler, “Red Cell metabolism. A manual of biochemical methods” eds. Grune & Stratton (1975).Google Scholar
  16. 16.
    D. L. Drabkin, Spectrophotometric studies. XV. Hydration of macro-sized crystals of human hemoglobin and osmotic concentration in red cells, J. Biol. Chem. 185:231 (1950).PubMedGoogle Scholar
  17. 17.
    V. Micheli, S. Sestini, P. Lusini, M. L. Ceccuzzi, C. Ricci, Reflections on the relevance of pyridine nucleotide synthesis in human circulating erythrocytes. “Macromolecules in the functioning cell” Proceedings of the 5th Soviet-Italian Symposium (Roma, 1988).Google Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Vanna Micheli
    • 1
  • Silvia Sestini
    • 1
  • Marina Rocchigiani
    • 1
  • Monica Pescaglini
    • 1
  • Carlo Ricci
    • 1
  1. 1.Istituto di Chimica BiologicaUniversità degli Studi di SienaItaly

Personalised recommendations