Advertisement

Genetics of Histone-Like Protein H-NS/H1 and Regulation of Virulence Determinants in Enterobacteria

  • Bernt Eric Uhlin
  • Björn Dagberg
  • Kristina Forsman
  • Mikael Göransson
  • Birgit Knepper
  • Peter Nilsson
  • Berit Sondén
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 58)

Abstract

Isolates of E. coli associated with intestinal or with extraintestinal disease are often characterized by their ability to express different properties thought to contribute to bacterial virulence. Examples of such properties are the synthesis of various types of adhesins, production of different kinds of cytotoxins (e.g. enterotoxins and hemolysins), ability to invade host tissue cells, and expression of certain capsule- and O-antigens. Changes in growth conditions may profoundly affect how different virulence-associated properties are expressed. The type of growth substrate, osmolarity, and growth temperature are examples of environmental factors found to influence the phenotypic expression of some of the virulence associated properties monitored under laboratory conditions. We have studied the expression of E. coli adhesins typically produced by many isolates from urinary tract infections in man. The P-specific adhesins are fimbrial adhesins, also referred to as pili, and they mediate binding to α-D-Gal-(1–4)-β-D-Gal-containing glycolipid structures. Genetic studies of the pap gene cluster have revealed functions of several of the gene products required for biogenesis of this type of adhesin (see Tennent et al., 1990 for a recent review).

Keywords

Serratia Marcescens Virulence Determinant lacZ Fusion Swedish Natural Science Research Council Extraintestinal Disease 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Båga, M., Göransson, M., Normark, S., and Uhlin, B.E., 1985. Transcriptional activation of a Pap pilus virulence operon from uropathogenic Escherichia coli, EMBO J., 4:3887PubMedGoogle Scholar
  2. Defez, R., and DeFelice, M., 1981, Cryptic operon for β-glucoside metabolism in Escherichia coli: genetic evidence for a regulatory protein, Genetics, 97 11.PubMedGoogle Scholar
  3. Diderichsen, B., 1980, cur-1, a mutation affecting the phenotype of sup+ strains of Escherichia coli, Mol. Gen. Genet., 180:425.PubMedCrossRefGoogle Scholar
  4. Drlica, K., and Rouviere-Yaniv, J., 1987, Histonelike proteins of bacteria. Microbiol. Rev., 51:301.PubMedGoogle Scholar
  5. Forsman, K., Göransson, M., and Uhlin, B.E., 1989. Autoregulation and multiple DNA interactions by a transcriptional regulatory protein in E. coli pili biogenesis, EMBO J., 8:1271.PubMedGoogle Scholar
  6. Göransson, M., and Uhlin, B.E., 1984, Environmental temperature regulates transcription of a virulence pili operon in E. coli, EMBO J., 3:2885.PubMedGoogle Scholar
  7. Göransson, M., Forsman, K., and Uhlin, B.E., 1988, Functional and structural homology among regulatory cistrons of pili-adhesin determinants in Escherichia coli, Mol. Gen. Genet., 212:412.PubMedCrossRefGoogle Scholar
  8. Göransson, M., Forsman, K., and Uhlin, B.E., 1989a, Regulatory genes in the thermoregulation of Escherichia coli pili gene transcription, Genes & Dev., 3:123.CrossRefGoogle Scholar
  9. Göransson, M., Forsman, K., Nilsson, P., and Uhlin, B.E., 1989b, Upstream activating sequences that are shared by two divergently transcribed operons mediate cAMP-CRP regulation of pilus-adhesin in Escherichia coli, Mol. Microbiol., 3:1557.PubMedCrossRefGoogle Scholar
  10. Göransson, M., Sondén, B., Nilsson, P., Dagberg, B., Forsman, K., Emanuelsson, K., and Uhlin, B.E., 1990, Transcriptional silencing and thermoregulation of gene expression in Escherichia coli, Nature, 344:682.PubMedCrossRefGoogle Scholar
  11. Higgins, C.F., Dorman, C.J., Stirling, D.A., Waddell, L., Booth, I.R., May, G., and Bremer, E., 1988, A physiological role for DNA supercoiling in the osmotic regulation of gene expression in S. typhimurium and E. coli, Cell, 52:569.PubMedCrossRefGoogle Scholar
  12. Hromockyj, A.E., and Maurelli, A.T., 1989, Identification of an Escherichia coli gene homologous to virR, a regulator of Shigella virulence, J. Bacteriol., 171:2879.PubMedGoogle Scholar
  13. Kohara, Y., Akiyama, K., and Isono, K., 1987, The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library, Cell, 50:495.PubMedCrossRefGoogle Scholar
  14. La Teana, A., Falconi, M., Scarlato, V., Lammi, M., and Pon, C.L., 1989. Characterization of the structural genes for the DNA-binding protein H-NS in Enterobacteriaceae, FEBS Lett., 244:34.PubMedCrossRefGoogle Scholar
  15. Marsh, M., and Hillyard, D.R., 1990, Nucleotide sequence of hns encoding the DNA-binding protein H-NS of Salmonella typhimurium, Nucleic Acids Res., 18:3397.PubMedCrossRefGoogle Scholar
  16. Maurelli, A.T., and Sansonetti, P.J., 1988, Identification of a chromosomal gene controlling temperature-regulated expression of Shigella virulence, Proc. Natl. Acad. Sci. USA, 85:2820.PubMedCrossRefGoogle Scholar
  17. Tennent, J.M., Hultgren, S., Marklund, B.-I., Forsman, K., Göransson, M., Uhlin, B.E., and Normark, S., 1990, Genetics of adhesin expression in Escherichia coli, in: “The Bacteria, Volume XI, Molecular basis of bacterial pathogenesis” B.H. Iglewski, and V.L. Clark, ed., Academic Press, Inc., San Diego.Google Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Bernt Eric Uhlin
    • 1
  • Björn Dagberg
    • 1
  • Kristina Forsman
    • 1
  • Mikael Göransson
    • 1
  • Birgit Knepper
    • 1
  • Peter Nilsson
    • 1
  • Berit Sondén
    • 1
  1. 1.Department of MicrobiologyUniversity of UmeåUmeåSweden

Personalised recommendations