A System for Production and Rapid Purification of Large Amounts of the Shiga Toxin/Shiga-Like Toxin B Subunit
Shiga toxin produced by Shigella dysenteriae type 1 is a potent protein toxin which has cytotoxic, neurotoxic and enterotoxic properties. The toxin inhibits eukaryotic protein synthesis by acting as an N-glycosidase, cleaving an adenine residue at nucleotide position 4324 of the 28S rRNA of the 60S ribosomal subunit. Some strains of Escherichia coli produce toxins which are biologically similar to Shiga toxin and are termed Shiga-like toxins (SLTs).
KeywordsHeLa Cell Shiga Toxin Adenine Residue Plasmid Expression Vector Periplasmic Extract
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