Comparison of Virulence Factors in Different Freshly Isolated Strains of Entmaoeba Histolytica

  • G. D. Burchard
  • D. Mirelman
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 58)


To produce liver abscesses, virulent Entamoeba histolytica have to cope with several problems: colonization of the gut, adherence to colonic epithelium, destruction of epithelial layer, adherence to and lysis of host inflammatory cells, and resistence to immune defence mechanisms. E. histolytica trophozoites obtained from symptomatic patients (designated as pathogenic) have been found to differ from those isolated from asymptomatic carriers (nonpathogenic) by a number of biochemical criteria such as isoenzyme electromigration, interaction with monoclonal antibodies or hybridization with DNA probes (Bracha et al. 1990). The reason for the difference in the pathogenic behaviour of the two classes of ameba is, however, not well understood, partly because only few studies were performed with nonpathogenic strains which cannot be grown in axenic cultures.


Methylene Blue Asymptomatic Carrier Trypanosoma Cruzi Adherence Mechanism Entamoeba Histolytica 
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  1. Bracha, R., Diamond, L.S., Ackers, J.P., Burchard, G.D., Mirelman, D.: Differentiation of clinical isolates of Entamoeba histolyt ica using specific DNA probes. J. Clin. Microbiol. 28 (1990) 680–684PubMedGoogle Scholar
  2. Burchard, G.D., Mirelman, D.: Entamoeba histolytica: virulence potential and sensitivity to Metronidazole and Emetine of four isolates possessing nonpathogenic zymodemes. Exp. Parasitol. 66 (1988) 231–242PubMedCrossRefGoogle Scholar
  3. Newman, S.I., Bucher, C., Rhodes, J., Bullock, W.E.: Phagocytosis of Histoplasma capsulatum yeasts and microconidia by human cultured macrophages and alveolar macrophages. Cellular cytoskeleton requirement for attachment and ingestion. J. Clin. Invest. 85 (1990) 223–230PubMedCrossRefGoogle Scholar
  4. Ouaissi, M.A., Cornette, J., Afchain, D., Capron, A., Gras-Masse, H., Tartar, A.: Trypanosoma cruzi infection inhibited by peptides modeled from a fibronectin cell attachment domain. Science 234 (1986) 603–607PubMedCrossRefGoogle Scholar
  5. Orozco, E., Rodriquez, M.A., Murphy, C.F., Salata, R. A., Petri, W.A., Smith, R.D., Ravdin, J.I.: Entamoeba histolytica: Cytopathogenicity and lectin activity of avirulent mutants. Exp. Parasitol. 63 (1987) 157–165PubMedCrossRefGoogle Scholar
  6. Orozco, E., Martinez Palomo, A., Robles, A.G., Guarneros, G., Galindo, J.M.: Las interacciones entre lectina y receptor median la adherencia de e. histolytica a células epiteliales. Relación de la adhesión con la virulencia de las cepas. Arch. Invest. Méd. (Mèx.) 13 (Supl.3) (1982) 159–167Google Scholar
  7. Petri, W.A., Chapman, M.D., Snodgrass, T., Mann, B.J., Broman, J., Ravdin, J.I.; Subunit structure of the galactose and N-acetyl-D-galactosamine-inhibitable adherence lectin of Entmoeba histolytica. J. Biochem. Chem. 264 (1989) 3007–3012Google Scholar
  8. Ravdin J.I., Guerrant, R.L.: The role of adherence in cytopathogenic mechanisms of E. histolytica. Study with mammmalian tissue culture cells and human red blood cells. J. Clin. Invest. 68 (1981) 1305–1313PubMedCrossRefGoogle Scholar
  9. Talamas-Rohana, P., Meza, I.: Interaction between pathogenic amebas and fibronectin: substrate degradation and changes in cytoskeleton organization. J. Cell Biol. 106 (1988) 1787–1794PubMedCrossRefGoogle Scholar
  10. Trissl, D., Martinez Palomo, A., de la Torre, M., de la Hoz, R., Perez de Suarez, E.: Surface properties of Entamoeba: increased rates of human erythrocyte phagocytosis in pathogenic strains. J. Exp. Med. 148 (1978) 1137–1145PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • G. D. Burchard
    • 1
  • D. Mirelman
    • 2
  1. 1.Bernhard-Nocht-Institute for Tropical MedicineHamburg 36Germany
  2. 2.Department of BiophysicsThe Weizmann Institute of ScienceRehovotIsrael

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