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DNA Probes and PCR Analysis in the Detection of Clostridium difficile and Helicobacter pylori

  • Brendan W. Wren
  • Christopher L. Clayton
  • Soad Tabaqchali
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 58)

Abstract

We have undertaken the molecular characterisation of a variety of species-specific antigens and potential virulence determinants from the enteric pathogens Clostridium difficile and Helicobacter pylori. The cloning and sequencing of these determinants has allowed the construction of nucleic acid probes and the synthesis of oligonucleotide primers used in PCR analysis for the sensitive and specific detection of these organisms.

Keywords

Polymerase Chain Reaction Analysis Clostridium Difficile Pylorus Strain HindIII Fragment Toxigenic Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    B. W. Wren, C. L. Clayton, P. Mullany, and S. Tabaqchali, Molecular cloning and expression of the Clostridium difficile toxin A gene in E. coli K-12. FEBS. 225: 82–86 (1987).CrossRefGoogle Scholar
  2. 2.
    B. W. Wren, C. L. Clayton, N. Castledine and S. Tabaqchali, Identification of toxigenic Clostridium difficile strains using a toxin A gene-specific probe. J Clin Micro 28: 1808–12 (1990).Google Scholar
  3. 3.
    B. W. Wren, C. L. Clayton, and S. Tabaqchali, Nucleotide sequence of Clostridium difficile toxin A gene fragment and detection of toxigenic strains by PCR. FEMS. 70: 1–6 (1990).CrossRefGoogle Scholar
  4. 4.
    B. W. Wren, N. B. Castledine and S. Tabaqchali, A sensitive oligonucleotide probe for the specific identification of toxigenic Clostridium difficile. J Med Micro 31: xvii (1990).Google Scholar
  5. 5.
    B. W. Wren, C. L. Clayton, and S. Tabaqchali, Rapid identification of toxigenic Clostridium difficile by PCR. Lancet 423, 335 (1990).Google Scholar
  6. 6.
    B. W. Wren, M. J. Pallen, and S. Tabaqchali, Beneficial effects of jumping PCR in diagnostic procedures. Lancet 1540, 335 (1990).Google Scholar
  7. 7.
    C. L. Clayton, B. W. Wren, P. Mullany, A. Topping, and S. Tabaqchali, Molecular cloning and expression of Campylobacter pylori species-specific antigens in E. coli K-12. Infection and Immunity, 57: 623–629 (1989).PubMedGoogle Scholar
  8. 8.
    C. L. Clayton, H. Kleanthous, B. W. Wren, P. Mullany, A. Topping, and S. Tabaqchali, Molecular cloning and characterisation of Campylobacter pylori species-specific antigens in E. coli K-12, in Gastroduodenal pathology and Campylobacter pylori, F. Megraud, ed., Elsevier, Amsterdam (1990).Google Scholar
  9. 9.
    C. L. Clayton, M. J. Pallen, H. Kleanthous, B. W. Wren, and S. Tabaqchali, Nucleotide sequence of urease genes from Helicobacter pylori encoding for urease subunits, Nucleic acids reearch, 18: 362 (1990).CrossRefGoogle Scholar
  10. 10.
    C. L. Clayton, M. J. Pallen, H. Kleanthous, B. W. Wren, and S. Tabaqchali, Molecular cloning and sequencing of Helicobacter pylori urease genes and detection of H. pylori using PCR. encoding for urease subunits, in Helicobacter pylori, Gastritis and peptic ulcer, P. Malfertheiner and H. Ditschuneit, eds., Springer-Verlag, Berlin (1990).Google Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Brendan W. Wren
    • 1
  • Christopher L. Clayton
    • 1
  • Soad Tabaqchali
    • 1
  1. 1.Department of Medical MicrobiologySt. Bartholomew’s Hospital Medical CollegeLondonUK

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