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Plasmid Encoded Virulence of Yersinia

  • H. Wolf-Watz
  • Å. Forsberg
  • R. Rosqvist
  • I. Bölin
  • K. Erickson
  • L. Norlander
  • M. Rimpiläinen
  • T. Bergman
  • S. Håkansson
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 58)

Abstract

The three virulent members of the genus Yersinia harbour related virulence Plasmids with a molecular weight of about 60-70 kb (11). When exponential phase cultures of these organisms growing in a Ca2+ free medium are shifted from 26C to 37C, growth ceases over a period of about 2 generations (10). If however 2.5 mM Ca2+ is present in the medium growth continues normally. These bacteria are referred to as being Ca2+ dependent (CD). Plasmid cured strains, however, do not show this dependency on Ca2+ and are thus, Ca2+ independent (CI). Such bacteria are always avirulent. By transposon insertion mutagenesis a 20 kb region of the virulence plasmid has been identified which is involved in this low calcium response (1cr). Such CI-mutants do not require Ca2+ for prolonged growth at 37C and they are not virulent. Although the plasmids of Y. enterocolitica. Y. pestis and Y. pseudotuberculosis have been subjected to rearrengements, the Ca2+ region, however, of the different plasmids is conserved (11).

Keywords

Virulence Determinant Yersinia Pestis Virulence Plasmid Exponential Phase Culture Tyrosine Phosphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Bölin, I., Å. Forsberg, L. Norlander, M. Skurnik and H. Wolf-Watz. 1988. Identification and mapping of temperature-inducible, plasmid encoded proteins of Yersinia spp.. Infect.Immun 56: 343–348.PubMedGoogle Scholar
  2. 2.
    Bölin, I., D. Portnoy and H. Wolf-Watz. 1985. Expression of the temperature-inducible outer membrane proteins of Yersinia. Infect.Immun. 48: 234–240.PubMedGoogle Scholar
  3. 3.
    Bölin, I. and H. Wolf-Watz. 1988. The plasmid encoded Yop2b protein of Yersinia pseudotuberculosis is a virulence determinant regulated by calcium and temperature at the level of transcription. Mol.Microbiol. 2: 237–245.PubMedCrossRefGoogle Scholar
  4. 4.
    Cornells, G., C. Sluiters, C. Lambert de Rouvroit and T. Michels. 1989. Homology between virF, the transcriptional activator of the Yersinia virulence regulon and araC. the Escherichia coli arabinose operon regulator. J.Bacteriol. 171: 254–262.Google Scholar
  5. 5.
    Forsberg, Å., I. Bölin, L. Norlander and H. Wolf-Watz. 1987. Molecular cloning and expression of calcium-regulated, plasmid coded proteins of Y. pseudotuberculosis. Microb.Pathog. 2: 123–137.PubMedCrossRefGoogle Scholar
  6. 6.
    Forsberg, Å. and H. Wolf-Watz. 1988. The virulence protein Yop5 of Yersinia pseudotuberculosis is regulated at transcriptional level by plasmid pIBl-encoded transacting elements controlled by temperature and calcium. Mol.Microbiol. 2: 121–133.PubMedCrossRefGoogle Scholar
  7. 7.
    Guan, K. and J. Dixon. 1990. Protein tyrosine phosphate activity of an essential virulence determinant in Yersinia. Science 249. 553–556.PubMedCrossRefGoogle Scholar
  8. 8.
    Leung, K. and S. Straley. 1989. The yopM gene of Yersinia pestis encodes a released protein having homology with the human patlet surface protein GPIba. J.Bacteriol. 171: 4623–4632.PubMedGoogle Scholar
  9. 8.
    Mulder, B., T. Michels, M. Simonet, M-P. Sory and G. Cornells. 1989. Identification of additional virulence determinants on the pYV plasmid of Yersinia enerocolitica 227. Infect.Immun. 57: 2534–2541.PubMedGoogle Scholar
  10. 9.
    Kupferberg, L. and K. Higuchi. 1958. Role of calcium ions in the stimulation of growth of virulent strains of Pasturella pestis. J. Bacteriol. 76: 120–121.PubMedGoogle Scholar
  11. 10.
    Portnoy, D., H. Wolf-Watz, I. Bölin, A. Beeder and S. Falkow. 1984. Characterization of common virulence plasmids in Yersinia species and their role in the expression of the outer membrane proteins. Infect.Immun. 43:108–114.PubMedGoogle Scholar
  12. 11.
    Rosqvist, R., Å. Forsberg, M. Rimpiläinen, T. Bergman and H. Wolf-Watz. 1990. The cytotoxic protein YopE of Yersinia obstructs the primary host defence. Molec.Microbiol. 4: 657–667.CrossRefGoogle Scholar
  13. 12.
    Rosqvist, R., I. Bölin and H. Wolf-Watz. 1988. Inhibition of phagocytosis in Yersinia pseudotuberculosis: a virulence plasmid encoded ability involving the Yop2b protein. Infect.Immun. 56: 2139–2143.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • H. Wolf-Watz
    • 1
    • 2
  • Å. Forsberg
    • 1
    • 2
  • R. Rosqvist
    • 1
    • 2
  • I. Bölin
    • 1
    • 2
  • K. Erickson
    • 1
    • 2
  • L. Norlander
    • 1
    • 2
  • M. Rimpiläinen
    • 1
    • 2
  • T. Bergman
    • 1
    • 2
  • S. Håkansson
    • 1
    • 2
  1. 1.Department of Applied Cell and Molecular BiologyUniversity of UmeåUmeåSweden
  2. 2.Department of Cell and Microbiology, FOA4University of UmeåUmeåSweden

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