Molecular Analysis of Potential Adhesions of Vibrio cholerae 01
Vibrio cholerae 01 is a non-invasive enteric pathogen of humans responsible for asiatic cholera. Thus, in order for it to cause disease it must adhere to and colonize the small intestinal mucosa where it is then able to efficiently deliver its toxins responsible for inducing the typical watery diarrhoea. The adhesions, colonization factors and toxins all have been the subject of interest because of the implications for vaccine development.
KeywordsProtective Antigen Colonization Factor Potential Adhesion Infant Mouse Kanamycin Resistance Cassette
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- 2.Chitnis, D.S., Sharma, K.D. and Kamat, R.S., 1982, Role of somatic antigen of Vibrio cholerae in adhesion to intestinal mucosa. J. Med. Microbiol., 5:52.Google Scholar
- 4.Franzon, V.L. and Manning, P.A., 1986, Molecular cloning and expression in Escherichia coli K-12 of the gene for a hemagglutinin from Vibrio cholerae. Infect. Immun., 52:279.Google Scholar
- 5.Guidolin, A. and Manning, P.A., 1985, Bacteriophage CP-T1 of Vibrio cholerae: identification of the cell surface receptor. Eur. J. Biochem., 153:89.Google Scholar
- 7.Hanne, L.F. and Finkelstein, R.A., 1982, Characterization and distribution of the hemagglutinins produced by Vibrio cholerae. Infect. Immun., 36:209.Google Scholar
- 8.Hantke, K., 1982, Negative control of iron uptake systems in Escherichiacoli. FEMS Microbiol. Lett., 15:83.Google Scholar
- 9.Kabir, S. and Showkat, A., 1983, Characterization of surface properties of Vibrio cholerae. Infect. Immun., 39:1048.Google Scholar
- 10.Manning, P.A., Heuzenroeder, M.W., Yeadon, J., Leavesley, D.I., Reeves, P.R. and Rowley, D., 1986, Molecular cloning and expression in Escherichia coJiK-122 of the CD-antigens of the Inaba and Ogawa serotypes of the Vibrio cholerae 01 lipopolysaccharides and their potential for vaccine development. Infect. Immun., 53:272.Google Scholar
- 11.Neoh, S.H. and Rowley, D., 1970, The antigens of Vibrio cholerae involved in vibriocidal action of antibody and complement. J. Infect. Dis., 121:505.Google Scholar
- 12.Sharma, D.P., Stroeher, U.H., Thomas, C.J., Manning, P.A. and Attridge, S.R., 1989a, The toxin-coregulated pilus (TCP) of Vibrio cholerae: molecular cloning of genes involved in pilus biosynthesis and evaluation of TCP as a protective antigen in the infant mouse model. Microbial Pathogènes is, 7:437.CrossRefGoogle Scholar
- 14.Taylor, R.K., Miller, V.L., Furlong, D.B. and Mekalanos, J.J., 1987, Use of phoA fusions to identify a pilus colonization factor coordinately regulated with cholera toxin. Proc. Natl. Acad. Sci. USA, 84:2833.Google Scholar
- 15.Tweedy, J.M., Park, R.W.A. and Hodgkiss, W., 1968, Evidence for the presence of fimbriae (pili) on vibrio species. J. Gen. Microbiol., 51:235.Google Scholar
- 17.Ward, H.M. and Manning, P.A., 1989, Mapping of chromosomal loci associated with lipopolysaccharide synthesis and serotype specificity in Vibrio cholerae 01 by transposon mutagenesis using Tn5 and Tn2680. Mol. Gen. Genet., 218:367.Google Scholar