Function and Molecular Architecture of E.Coli Adhesins
The pathogenicity of E.coli has been ascribed to different virulence factors such as toxins, cytolysins, serum resistance, O- and K-antigens or adhesive properties. The adherence of the bacteria to epithelial surfaces — e.g. cell membranes and/or mucosal surfaces — is an important early event in host parasite interactions leading to bacterial colonization and infection (1). The term adhesion as used in this context describes a specific interaction of recognition proteins attached to the bacterial surface with complex carbohydrate moieties of glycoproteins and/or glycolipids on mammalian cells. These recognition proteins (also termed adhesins, hemagglutinins or lectins) may have different appearances in the electron microscope. Structures that can be demonstrated directly by negative staining procedures have been termed fimbriae or pili (rigid, 5–7 nm diameter) and fibrillae (flexible, 2–3 nm diameter). Nonfimbrial adhesive structures can only be visualized after stabilization with specific antibodies and then have a capsule like appearance (2).
KeywordsAdhesive Property Molecular Architecture Receptor Binding Site Minor Protein Host Parasite Interaction
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- (6).Schmoll, T., Hoschützky, H., Morschhäuser, J., Lottspeich, F., Jann, K., and Hacker, J. (1989). Analysis of genes coding for the sialic acid-binding adhesin and two other minor fimbrial subunits of the S fimbrial adhesin determinant of Escherichia coli. Mol. Microbiol. 3:1735–1744.PubMedCrossRefGoogle Scholar
- (7).Riegman, N., Hoschützky, H., van Die, I., Hoekstra, W., Jann, K, and Bergmans, H., (1990). Immunochemical analysis of P-fimbrial structure: localization of the minor subunits and the influence of the minor subunit FsoE on the biogenesis of the adhesin. Mol. Microbiol. 4:1193–1198.PubMedCrossRefGoogle Scholar
- (8).Goldhar, J., Perry, R., Golecki, J.R., Hoschützky, H., Jann, B. and Jann, K. (1989). Non-fimbrial, mannose-resi-stant adhesins from uropathogenic Escherichia coli 083:K1:H4 and 014:K?:H11. Infect. Immun. 55:1837–1842.Google Scholar