Advertisement

Biodegradation of Mixed Solvents by a Strain of Pseudomonas

  • J. C. Spain
  • C. A. Pettigrew
  • B. E. Haigler
Part of the Environmental Science Research book series (ESRH, volume 41)

Abstract

Substituted aromatic compounds are used extensively as solvents, synthetic intermediates, pesticides, and fuels. They are released in the environment in tremendous quantities and can pose a considerable human health hazard, particularly in groundwater. The most common groundwater contamination problem in the United States is caused by gasoline components such as benzene, toluene, ethylbenzene, and xylenes leaking from underground storage tanks. These and other petroleum components in gasoline are readily biodegradable and bioremediation is often the treatment of choice for cleanup. Indigenous mixed microbial communities are typically able to carry out the treatment process if supplied with oxygen and mineral nutrients. Biotreatment has been successful for petroleum hydrocarbons in groundwater and soil (Thomas and Ward, 1989) and in landfarming (Bartha and Bossert, 1984).

Keywords

Aromatic Compound Mixed Solvent Muconic Acid Underground Storage Tank Broad Substrate Range 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alexander, M., 1979, Role of cometabolism, in: Microbial Degradation of Pollutants in Marine Environments: Proceedings of the Workshop, (A.W. Bourquin and P.H. Pritchard, eds.) U.S. Environmental Protection Agency, EPA-600/9–79–012, pp.67–75.Google Scholar
  2. Bartha, R. and Bossert, I., 1984, The treatment and disposal of petroleum wastes, in: Petroleum Microbiology, (R.M. Atlas, ed.), Macmillan Publishing Company, New York, New York, pp. 553–577.Google Scholar
  3. Dagley, S., and Gibson, D.T., 1965, The bacterial degradation of catechol, Biochem. J. 85:466–474.Google Scholar
  4. Dorn, E., and Knackmuss, H.-J., 1978, Chemical structure and biodegradability of halogenated aromatic compounds. Two catechol 1,2-dioxygenases from a 3-chlorobenzoate-grown pseudomonad, Biochem. J. 174:73–84.PubMedGoogle Scholar
  5. Evans, W.C., Smith, B.S.W., Fernley, H.N., and Davies, J.I., 1971, Bacterial metabolism of 2,4-dichlorophenoxyacetate, Biochem. J. 122:543–551.PubMedGoogle Scholar
  6. Frick, T.D., Crawford, R.L., Martinson, M., Chresand, T., and Bateson, G., 1988, Microbiological cleanup of groundwater contaminated by pentachlorophenol, in: Environmental Biotechnology: Reducing Risks from Environmental Chemicals Through Biotechnology, (G.S. Omenn, ed.), Plenum Press, New York, New York, pp. 173–192.Google Scholar
  7. Gibson, D.T., and Subramanian, V., 1984, Microbial degradation of hydrocarbons, in: Microbial Degradation of Organic Compounds, (D.T. Gibson, ed.) Marcel Dekker, Inc., New York, pp. 181–252.Google Scholar
  8. Gibson, D.T., Zylstra, G.J., and Chauhan, S., 1990, Biotransformations catalyzed by toluene dioxygenase from Pseudomonas putida F1, in: Pseudomonas: Biotransformations, Pathogenesis, and Evolving Biotechnology, (S. Silver, A.M. Chakrabarty, B. Iglewski, and S. Kaplan, eds.), American Society for Microbiology, Washington, D.C., pp. 121–132.Google Scholar
  9. Haigler, B.E., and Spain, J.C., 1989, Degradation of p-chlorotoluene by a mutant of Pseudomonas sp. Strain JS6, Appl. Environ. Microbiol. 55:372–379.PubMedGoogle Scholar
  10. Knackmuss, H.-J., 1981, Degradation of halogenated and sulfonated hydrocarbons, in: Microbial Degradation of Xenobiotics and Recalcitrant Compounds, (T. Leisinger, R. Hutter, A.M. Cook, and J. Nuesch, eds.), Academic Press, London, pp. 190–212.Google Scholar
  11. Morgan, P., and Watkinson, R.J., 1989, Microbiological methods for the cleanup of soil and ground water contaminated with halogenated organic compounds, FEMS Microbiol. Rev. 63:277–300.CrossRefGoogle Scholar
  12. Ornston, L.N., and Stanier, R. Y., 1966, The conversion of catechol and protocatechuate to B-keto adipate by Pseudomonas putida-biochemistry, J.Biol. Chem.241:3776–3786.PubMedGoogle Scholar
  13. Reineke, W., and Knackmuss, H.-J., 1984, Microbial metabolism of haloaromatics: isolation and properties of a chlorobenzene-degrading bacterium, Appl. Environ. Microbiol. 47:395–402. PubMedGoogle Scholar
  14. Rojo, F., Pieper, D.H., Engesser, K.-H., Knackmuss, H.-J., and Timmis, K.T., 1987, Assemblage of ortho cleavage route for simultaneous degradation of chloro- and methylaromatics, Science 238:1395–1398.PubMedCrossRefGoogle Scholar
  15. Spain, J.C., and Nishino, S.F., 1987, Degradation of 1,4-dichlorobenzene by a Pseudomonas sp., Appl Environ. Microbiol. 53:1010–1019.PubMedGoogle Scholar
  16. Taeger, K., Knackmuss, H.-J., and Schmidt, E., 1988, Biodegradability of mixtures of chloro- and methylsubstituted aromatics: Simultaneous degradation of 3-chlorobenzoate and 3-methylbenzoate, Appl Microbiol. Biotechnol. 28:603–608.CrossRefGoogle Scholar
  17. Thomas, J.M., Ward, C.H., 1989, In situ biorestoration of organic contaminants in the subsurface, Environ. Sci Technol. 23:760–766.CrossRefGoogle Scholar
  18. Wilson, J.T., and Wilson, B.H., 1985, Biotransformation of trichloroethylene in soil, Appl. Environ. Microbiol. 49:242–243.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • J. C. Spain
    • 1
  • C. A. Pettigrew
    • 1
  • B. E. Haigler
    • 1
  1. 1.Tyndall AFBU.S. Air Force Engineering and Services CenterUSA

Personalised recommendations