On the Disposition of De Novo Synthesized Quinolinic Acid in Rat Brain Tissue
Because of the putative role of the cerebral metabolite and specific N-methyl-D-aspartate receptor agonist quinolinic acid (QUIN) in physiological and pathological processes in the brain (Schwarcz et al., 1984; Stone and Burton, 1989; Lapin et al., this volume), the mechanisms by which QUIN function is regulated in the normal and diseased brain have become the object of investigative interest. The presence of 3-hydroxyanthranilic acid oxygenase (3HA0), QUIN’s immediate biosynthetic enzyme, in brain tissue (Foster et al., 1986b) indicated the possibility to assess the dynamics of QUIN disposition in whole cell preparations (tissue slices) which had been exposed to the substrate of 3HA0, 3-hydroxyanthranilic acid (3HANA). Preliminary experiments showed that QUIN can indeed be recovered from both tissue and incubation medium after the administration of 3HANA to slices from rat liver or brain in a physiological milieu (Speciale et al., 1989). Assay parameters have now been scrutinized in detail and optimal conditions for the examination of QUIN function in vitro have been established (Speciale et al., submitted for publication). In the present report, we describe the use of this experimental system for the initial assessment of mechanisms of QUIN release in normal and lesioned rat brain.
KeywordsQuinolinic Acid Extracellular Compartment Ibotenic Acid Striatal Slice Basal Ganglion Lesion
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