Kynurenine 3-Monooxygenase Activity of Rat Brain Mitochondria Determined by High Performance Liquid Chromatography with Electrochemical Detection
Among the enzymes of the kynurenine pathway in brain, the presence of the following five enzymes has been reported: IDO (Gál, 1974), kynurenine 3-monooxygenase (Battie and Verity, 1981), kynureninase (Kawai et al., 1988), 3-OHAADO (Foster et al., 1986) and QPRT (Foster et al., 1985)*. The reported specific activity of rat brain mitochondrial kynurenine 3-monooxygenase is rather higher than that of liver. We have now reexamined this enzyme activity in rat brain mitochondria by using a new, highly sensitive assay method (HPLC-ECD). The enzyme activity of rat brain mitochondria is lower than that of liver mitochondria.
KeywordsLiver Mitochondrion Quinolinic Acid Kynurenine Pathway HPLC Elution Membrane Electron Transport
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