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Brain Indole Metabolism Assessed Using in Vivo Dialysis

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Kynurenine and Serotonin Pathways

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 294))

Abstract

The relationships between tryptophan (Trp) and its major CNS metabolites serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) may be studied by analysis of post-mortem tissue or by analyzing concentrations in the extracellular fluid. Major advances in our understanding of the physiological importance of Trp supply to the brain and subsequent 5-HT synthesis and metabolism was derived, in part, from regional brain tissue analysis following e.g. Trp loading, stress, fasting (Fernstrom and Wurtman, 1971; Knott and Curzon, 1974; Curzon and Marsden, 1975). However, tissue analysis does not differentiate between functionally active substrate concentrations available to receptors in the extracellular compartment and intracellular concentrations. Furthermore, as only a single value is obtained from each animal, large numbers of animals are required to obtain temporal profiles of drug action. The primary advantage of in vivo monitoring of extracellular substrate concentrations is that repeated measurements may be made in the same animal over time. When applied to the conscious, freely moving animal, this allows associations between neurochemical changes and their roles in behaviors to be determined.

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  1. Institute of Neurology, London, WC1N 1PG, UK

    G. Sarna

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  1. G. Sarna
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  1. Maryland Psychiatric Research Center, University of Maryland School of Medicine, Baltimore, Maryland, USA

    Robert Schwarcz

  2. McGill University, Montreal, Quebec, Canada

    Simon N. Young

  3. University of Wisconsin, Madison, Wisconsin, USA

    Raymond R. Brown

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© 1991 Plenum Press, New York

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Sarna, G. (1991). Brain Indole Metabolism Assessed Using in Vivo Dialysis. In: Schwarcz, R., Young, S.N., Brown, R.R. (eds) Kynurenine and Serotonin Pathways. Advances in Experimental Medicine and Biology, vol 294. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5952-4_6

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  • DOI: https://doi.org/10.1007/978-1-4684-5952-4_6

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