Differential Effects of Estradiol and Hydrocortisone on Liver Tryptophan Pyrrolase in Rats of Various Ages

  • S. K. Patnaik
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 294)


Changes in the levels of certain regulatory enzymes and their indue-ibility by several hormones might account for the deterioration of physiological efficiency during aging in animals. Such changes may be brought about by the alterations in the template activity of their corresponding genes (Kanungo, 1975). Tryptophan pyrrolase (TP; L-tryptophan-oxygen 2, 3-oxidoreductase, E.C. is a liver cytosolic enzyme that catalyzes the first and rate-limiting step in the degradation of tryptophan (Knox and Mehler, 1950), resulting in the synthesis of nicotinamide cofactors (Nish-izuka and Hayaishi, 1963). The present paper describes the activity and induction pattern of liver TP by estradiol and hydrocortisone in rats of different ages to elucidate molecular mechanisms of aging.


Estradiol Treatment Template Activity Hydrocortisone Treatment Ornithine Aminotransferase Nicotinamide Cofactor 
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  1. Kanungo, M.S., 1975, A model for aging, J. Theor. Biol., 53: 253–261.PubMedCrossRefGoogle Scholar
  2. Kanungo, M.S., Patnaik, S.K., and Koul, O., 1975, Decrease in 17-beta-estra-diol receptor in brain of aging rats, Nature, 253: 366–367.PubMedCrossRefGoogle Scholar
  3. Knox, W.E., and Mehler, A.H., 1950, The conversion of tryptophan to kynur-enine in liver: the coupled tryptophan peroxidase system forming formyl-kynurenine, J. Biol. Chem., 187: 419–430.PubMedGoogle Scholar
  4. Knox, W.E., Yip, A., and Reshef, L., 1970, L-tryptophan 2, 3-di-oxygenase (tryptophan pyrrolase) (rat liver), Meth. Enzymol., 17a: 415–421.CrossRefGoogle Scholar
  5. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., 1951, Protein estimation with Folin-Ciocalteau reagent, J. Biol. Chem., 193: 265–275.PubMedGoogle Scholar
  6. Nishizuka Y., and Hayaishi, O., 1963, Studies on the biosynthesis of nico-tinamide adenine dinucleotide: enzymatic synthesis of niacin ribonucleo-tides from 3-hydroxyanthranilic acid in mammalian tissues, J. Biol. Chem., 238: 3369–3377.PubMedGoogle Scholar
  7. Patnaik, S.K., 1979, Changes in the sub-types of soluble alanine aminotrans-ferase in the liver of rats during development and aging, Cell Biol. Int. Rep., 7: 607–614.CrossRefGoogle Scholar
  8. Patnaik, S.K., 1989, Evidence for estradiol induced differential expression of genes for brain RNA polymerase during developmental stages of the rat, Biochem. Int., 18: 721–728.PubMedGoogle Scholar
  9. Patnaik, S.K., and Patnaik, R., 1989, Tissue-specific differential induction of arginase and ornithine aminotransferase by hydrocortisone during various developmental stages of the rat, Biochem. Int., 18: 709–719.PubMedGoogle Scholar
  10. Patnaik, R., Sarangi, S.K., and Patnaik, S.K., 1987a, Tissue-specific differential induction of ornithine aminotransferase by estradiol in rats of various ages, Biochem. Int., 14: 843–850.PubMedGoogle Scholar
  11. Patnaik, S.K., Sharma, R., and Patnaik, R., 1987b, Differential effects of hydrocortisone on aspartate aminotransferase isozymes of the liver of rats during growth, development, and senescence, Biochem. Int., 15: 611–617.PubMedGoogle Scholar
  12. Roth, G.S., and Adelman, R.C., 1974, Age-dependent regulation of mammalian DNA synthesis and cell division in vivo by glucocorticoids, Exp. Gerontol., 9: 27–31.PubMedCrossRefGoogle Scholar
  13. Roth, G.S., and Adelman, R.C., 1975, Age-related changes in hormone binding by target cells and tissues, possible role in altered adaptive responsiveness, Exp. Gerontol., 10: 1–11.PubMedCrossRefGoogle Scholar
  14. Sharma, R., and Patnaik, S.K., 1983, Induction of phosphoenolpyruvate carbo-xykinase by hydrocortisone in rat liver and brain as a function of age, Biochem. Int., 7: 535–540.PubMedGoogle Scholar
  15. Sharma, R., and Patnaik, S.K., 1984, Age-related response of citrate synthase to hydrocortisone in the liver and brain of male rats, Experientia, 40: 97–98.PubMedCrossRefGoogle Scholar
  16. Sharma, R., and Patnaik, S.K., 1985, Age-dependent regulation of aspartate aminotransferase isozymes by hydrocortisone in the brain of male rats, Mol. Physiol., 7: 195–200.Google Scholar
  17. Singer, S., Ito, H., and Litwack, G., 1973, 3H-Cortisol binding by young and old human liver cytosol protein in vitro. Int. J. Biochem., 4: 569–573.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • S. K. Patnaik
    • 1
  1. 1.Department of PharmaceuticsInstitute of Technology Banaras Hindu UniversityVaranasiIndia

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