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Induction of Indoleamine 2,3-Dioxygenase in Human Cells in Vitro

  • G. Werner-Felmayer
  • E. R. Werner
  • D. Fuchs
  • A. Hausen
  • G. Reibnegger
  • H. Wachter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 294)

Abstract

Human macrophages (MO) release neopterin and degrade tryptophan by in-doleamine 2, 3-dioxygenase (IDO), when stimulated with interferon (IFN)-gamma in vitro (Werner et al., 1987a). Also, in a panel of non-immune cells, induction of IDO by IFN-gamma is strictly correlated to pteridine synthesis, which results in intracellularly increased levels of biopterin and/or neopterin (for details see Werner et al., 1989 and this volume). Out of the peripheral blood mononuclear cell fraction, MO are the cell population responsible for formation of neopterin and for IDO activity (Huber et al., 1984; Werner et al., 1987a). Lymphocytes do not release neopterin and are not inducible for IDO.

Keywords

Total Cell Protein Neopterin Level Human Peripheral Blood Monocyte Tryptophan Metabolite Peripheral Blood Mononuclear Cell Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • G. Werner-Felmayer
    • 1
  • E. R. Werner
    • 1
  • D. Fuchs
    • 1
  • A. Hausen
    • 1
  • G. Reibnegger
    • 1
  • H. Wachter
    • 1
  1. 1.Institute of Medical Chemistry and BiochemistryUniversity of InnsbruckInnsbruckAustria

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