Induction of Indoleamine 2,3-Dioxygenase in Human Cells in Vitro
Human macrophages (MO) release neopterin and degrade tryptophan by in-doleamine 2, 3-dioxygenase (IDO), when stimulated with interferon (IFN)-gamma in vitro (Werner et al., 1987a). Also, in a panel of non-immune cells, induction of IDO by IFN-gamma is strictly correlated to pteridine synthesis, which results in intracellularly increased levels of biopterin and/or neopterin (for details see Werner et al., 1989 and this volume). Out of the peripheral blood mononuclear cell fraction, MO are the cell population responsible for formation of neopterin and for IDO activity (Huber et al., 1984; Werner et al., 1987a). Lymphocytes do not release neopterin and are not inducible for IDO.
KeywordsTotal Cell Protein Neopterin Level Human Peripheral Blood Monocyte Tryptophan Metabolite Peripheral Blood Mononuclear Cell Culture
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