Signal-to-Noise Optimization of HPLC-Fluorometric Systems and their Application to the Analysis of Indoles
The signal-to-noise optimization of high performance liquid Chromatographic (HPLC) flow-cell fluorometric systems is described and the possibilities for further improving limits of detection for indoles is discussed. Application of HPLC-fluorometry to analyses in rat cerebrospinal fluid (CSF), human lumbar CSF, and human blood is presented. Finally, the intriguing clinical finding of hyperserotonemia in autism is discussed.
KeywordsHigh Performance Liquid Chromatographic Electrophoresis Serotonin Arginine Tryptophan
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