Mass Spectrometric Determinations of Tryptophan and its Metabolites
The unique character of a chemical compound is determined by its molecular architecture. That architecture is a sum not only of all of the atoms in the compound and their masses, but of their particular relationship in space, i.e., the molecular bonds, sub-structural components, and their stereochemistry. Consequently, a physical tool which can measure both the summed masses of the elements in a compound and reflect the subtleties of their arrangement is very useful in both quantitative and qualitative investigations in biochemistry.
KeywordsQuinolinic Acid Kynurenic Acid Chemical Ionization Mass Spectrometry Tryptophan Metabolism Mass Spectrometric Determination
Unable to display preview. Download preview PDF.
- Caprioli, R.M., 1972, Use of stable isotopes, in: “Biochemical Applications of Mass Spectrometry”, G.R. Waller, ed., Wiley, New York.Google Scholar
- Curtius, H.-Ch., Farner-Wegmann, H., Niederwieser, A., and Rey, F., 1980, in vivo measurement of tryptophan-5-hydroxylase activity using stable isotopes and GC/MS, in: “Biochemical and Medical Aspects of Tryptophan Metabolism”, Hayaishi, O., Ishimura, Y., and Kido, R., eds., Elsevier/ North-Holland, Amsterdam, pp. 281–290.Google Scholar
- Davis, B. A., and Durden, D.A., 1987, A comparison of the gas Chromatographic and mass spectrometric properties of the pentafluoropropionyl and heptafluorobutyryl derivatives of the methyl, trifluoroethyl, pentafluoropropyl and hexafluoroisopropyl esters of twelve acidic metabolites of biogenic amines, Biomed. Environ. Mass. Spectrom., 14: 197–206.PubMedCrossRefGoogle Scholar
- Davis, B.A., Durden, D.A., and Boulton, A.A., 1986, Simultaneous analysis of twelve biogenic amine metabolites in plasma, cerebrospinal fluid and urine by capillary column gas chromatography-high-resolution mass spectrometry with selected-ion monitoring. J. Chromatogr., 374: 227–238.PubMedCrossRefGoogle Scholar
- Heyes, M.P., and Markey, S.P., 1988a, Quantification of quinolinic acid in rat brain, whole blood, and plasma by gas chromatography and negative chemical ionization mass spectrometry: effects of systemic L-tryptophan administration on brain and blood quinolinic acid concentrations, Anal. Biochem., 174: 349–359.PubMedCrossRefGoogle Scholar