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IGF-I Mediated Recruitment of Glucose Transporters from Intracellular Membranes to Plasma Membranes in L6 Muscle Cells

  • Philip J. Bilan
  • Toolsie Ramlal
  • Amira Klip
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 293)

Abstract

IGF-I has long been known to have mitogenic effects on isolated skeletal tissue (Salmon and Daughaday, 1957) and insulin-like metabolic effects on isolated adipose tissue and muscle tissue (Froesch et al., 1966; Poggi et al., 1979). Stimulation of glucose utilization by IGF-I has been observed in vivo in rats and humans (Zapf et al., 1986; Guler et al., 1987). Moreover, Giacca et al. (1990) have demonstrated that skeletal muscle is the preferred site for the stimulation of glucose utilization by IGF-I in completely insulin-deficient diabetic dogs. Simultaneously, Moxely III et al. (1990) demonstrated that in vivo, IGF-I stimulates hexose uptake directly into rat muscles. These specific effects of IGF-I on skeletal muscle are consistent with the observation that skeletal muscle expresses abundant amounts of IGF-I receptors (Livingston et al., 1988, Dohm et al., 1990), whereas adipose tissue has very low numbers of IGF-I receptors (Rechler and Nissley, 1985; Sinha et al., 1990). Skeletal muscle is also the primary site of action for the stimulation of glucose utilization by insulin in vivo (Defronzo et al., 1981). Although IGF-I and insulin have common biological actions, IGF-I and insulin receptors may function independently. For example, rat 1 fibroblasts expressing a mutant insulin receptor with an inactive tyrosine kinase are unresponsive to insulin stimulation of glucose uptake but can still respond to IGF-I through their endogenous IGF-I receptors (McClain et al., 1990). Secondly, Lammers et al. (1989) demonstrated that chimeric receptors consisting of the extracellular insulin receptor domain and the intracellular IGF-I receptor domain were ten times more responsive to insulin for stimulation of DNA synthesis than was the native insulin receptor. This suggested that the intracellular kinase of the IGF-I receptor is more active than the insulin receptor kinase and is thus inherently different from its insulin receptor counterpart. Hence, IGF-I and insulin and their receptors share common responses but can trigger them independently.

Keywords

Insulin Receptor Membrane Fraction Internal Membrane Plasma Membrane Fraction Plasma Membrane Marker 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Philip J. Bilan
    • 1
  • Toolsie Ramlal
    • 1
  • Amira Klip
    • 1
  1. 1.Division of Cell BiologyThe Hospital for Sick ChildrenTorontoCanada

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