The Effect of Quantity and Nutritional Quality of Dietary Proteins on Plasma Concentration of Insulin-Like Growth Factor Binding Proteins (IGFBP) and the Saturability of IGFBP with Endogenous IGF-I

  • Hisanori Kato
  • Tsutomu Umezawa
  • Yutaka Miura
  • Tadashi Noguchi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 293)


Plasma concentrations of insulin-like growth factor-I (IGF-I) have been shown to be controlled by growth hormone, insulin and nutritional status of animals.1–3 In previous papers, the present authors demonstrated that quantity and nutritional quality of dietary proteins significantly affect the plasma concentration of IGF-I.4 The concentration correlated well with the growth rate or the rate of whole body protein synthesis.5 We also showed that liver content of IGF-I mRNA was also strongly affected by the status of protein nutrition.6 7 It decreased greatly under protein deprivation and it was highly correlated with the plasma IGF-I concentration. The content of the mRNA species of larger molecular weight was most severely affected. These results strongly suggest that the rate of body protein synthesis or growth rate is regulated by the plasma concentration of IGF-I through liver IGF-I mRNA content (or the rate of synthesis and secretion of IGF-I by liver).


Casein Diet Isolate Soybean Protein Fractional Synthesis Rate Acid Labile Subunit Ligand Blotting 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    J. J. Van Wyk, The somatomedins: Biological actions and physiologic control mechanisms, in:“Hormonal Proteins and Peptides, vol. 12,” pp. 8 (1984).Google Scholar
  2. 2.
    E. R. Froesch, C. Schmid, J. Schwander, and J. Zapf, Actions of insulin-like growth factors. Ann. Rev. Physiol., 47:443 (1985).CrossRefGoogle Scholar
  3. 3.
    D. LeRoith, and M. K. Raizada, (eds.) “Molecular and Cellular Biology of Insulin-like Growth Factors and Their Receptors”, Plenum Press, New York and London (1989).Google Scholar
  4. 4.
    S. Takahashi, M. Kajikawa, T. Umezawa, S.-I. Takahashi, H. Kato, Y. Miura, T. J. Nam, T. Noguchi, and H. Naito, Effect of dietary proteins on the plasma immunoreactive insulin-like growth factor-1/somatomedin C concentration in the rat. Brit. J. Nutr. 63:521 (1990).PubMedCrossRefGoogle Scholar
  5. 5.
    T. J. Nam, T. Noguchi, R. Funabiki, H. Kato, Y. Miura, and H. Naito, Correlation between the urinary excretion of acid-soluble peptides, fractional synthesis rate of whole body proteins, and plasma immunoreactive insulin-like growth factor-1/somatomedin C concentration in the rat. Brit. J. Nutr. 63:515 (1990).PubMedCrossRefGoogle Scholar
  6. 6.
    H. Kato, Y. Miura, A. Okoshi, T. Umezawa, S.-I. Takahashi, and T. Noguchi, Dietary and hormonal factors affecting the mRNA level of IGF-I in rat liver in vivo and in primary cultures of rat hepatocytes. in:“Molecular and Cellular Biology of Insulin-like Growth Factors and Theier Receptors,” D. LeRoith, and M. K. Raizada, (eds.) Plenum Press, New York and London, pp.125, (1989).CrossRefGoogle Scholar
  7. 7.
    D. S. Straus, and C. D. Takemoto, Effect of protein deprivation on insulin-like growth factor(IGF)-I and -II, IGF binding protein-2, and serum albumin gene expression in rat. Endocrinology 127:1849 (1990).PubMedCrossRefGoogle Scholar
  8. 8.
    Y. W.-H. Yang, J.-F. Wang, C. C. Orlowski, S. P. Nissley, and M. M. Rechler, Structure, specificity, and regulation of the insulinlike growth factor-binding proteins in adult rat serum. Endocrinology 125:1540 (1989).PubMedCrossRefGoogle Scholar
  9. 9.
    T. Umezawa, Y. Ohsawa, Y. Miura, H. Kato, and T. Noguchi, Effect of protein deprivation on insulin-like growth factor binding proteins in rats. Brit. J. Nutr. in press.Google Scholar
  10. 10.
    P. Hossenlopp, D. Seurin, B. Segovia-Quinson, S. Hardouin, and M. Binoux, Analysis of serum insulin-like growth factor binding proteins using western blotting: Use of the method for titration of the binding proteins and competitive binding studies. Anal. Biochem. 154:138 (1986).PubMedCrossRefGoogle Scholar
  11. 11.
    S. Hardouin, P. Hossenlopp, B. Segovia, D. Seurin, G. Portolan, C. Lassarre, and M. Binoux, Heterogeneity of insulin-like growth factor binding proteins and relationships between structure and affinity. (1) Circulating forms in man. Eur. J. Biochem. 170:121 (1987).PubMedCrossRefGoogle Scholar
  12. 12.
    J. Zapf, W. Born, J.-Y. Chang, P. James, E. R. Froesch, and J. A. Fischer, Isolation and NH2-terminal amino acid sequences of rat serum carrier proteins for insulin-like growth factors. Biochem. Biophys. Res. Commun. 156:1187 (1988).PubMedCrossRefGoogle Scholar
  13. 13.
    R. C. Baxter, and J. L. Martin, Structure of the Mr 140,000 growth hormone-dependent insulin-like growth factor binding protein complex: Determination by reconstitution and affinity-labeling. Proc. Natl. Acad. Sci. USA. 86:6898 (1989).PubMedCrossRefGoogle Scholar
  14. 14.
    J. L. Martin, and R. C. Baxter, Insulin-like growth factor-binding protein from human plasma: Purification and characterization. J. Biol. Chem. 261:8754 (1986).PubMedGoogle Scholar
  15. 15.
    G. T. Ooi, and A. C. Herington, Recognition of insulin-like growth factor (IGF) serum binding proteins by an antibody raised against a specific IGF-inhibitor. Biochem. Biophys. Res. Commun. 156:783 (1988).PubMedCrossRefGoogle Scholar
  16. 16.
    W. H. Busby, D. K. Snyder, and D. R. Clemmons, Radioimmunoassay of a 26,000-dalton plasma insulin-like growth factor-binding protein: Control by nutritional variables. J. Clin. Endocrinol. Metab. 67:1225 (1988).PubMedCrossRefGoogle Scholar
  17. 17.
    S. I. Yeoh, and R. C. Baxter, Metabolic regulation of the growth hormone independent insulin-like growth factor binding protein in human plasma. Acta Endocrinol. 119:465 (1988).PubMedGoogle Scholar
  18. 18.
    R. H. McCusker, D. R. Campion, W. K. Jones, and D. R. Clemmons, The insulin-like growth factor-binding proteins of porcine serum: Endocrine and nutritional regulation. Endocrinology 125:501 (1989).PubMedCrossRefGoogle Scholar
  19. 19.
    K. Brismar, M. Gutniak, G. Povoa, S. Werner, and K. Hall, Insulin regulates the 35 kDa IGF binding protein in patients with diabetes mellitus. J. Endocrinol. Invest. 11:599 (1988).PubMedGoogle Scholar
  20. 20.
    A.-M. Suikkari, V. A. Koivisto, E.-M. Rutanen, H. Yki-Jarvinen, S.-L. Karonen, and M. Seppala, Insulin regulates the serum levels of low molecular weight insulin-like growth factor-binding protein. J. Clin. Endocrinol. Metab. 66:266 (1988).PubMedCrossRefGoogle Scholar
  21. 21.
    T. G. Unterman, D. T. Oehler, and R. E. Becker, Identification of a type 1 insulin-like growth factor binding protein (IGF BP) in serum from rats with diabetes mellitus. Biochem. Biophys. Res. Commun. 163:882 (1989).PubMedCrossRefGoogle Scholar
  22. 22.
    A.-M. Suikkari, V. A. Koivisto, R. Koistinen, M. Seppala, and H. Yki-Jarvinen, Dose-response characteristics for suppression of low molecular weight plasma insulin-like growth factor-binding protein by insulin. J. Clin. Endocrinol. Metab. 68:135 (1989).PubMedCrossRefGoogle Scholar
  23. 23.
    G. T. Ooi, C. C. Orlowski, A. L. Brown, R. E. Becker, T. G. Unterman, and M. M. Rechler, Different tissue distribution and hormonal regulation of messenger RNAs encoding rat insulin-like growth factor-binding proteins-1 and -2. Mol. Endocrinol. 4:321 (1990).PubMedCrossRefGoogle Scholar
  24. 24.
    L. J. Murphy, C. Seneviratne, G. Ballejot, F. Croze, and T. G. Kennedy, Identification and characterization of a rat decidual insulin-like growth factor-binding protein complementary DNA. Mol. Endocrinol. 4:329 (1990).PubMedCrossRefGoogle Scholar
  25. 25.
    National Research Council. Nutrient requirements of the laboratory rat, in:“Nutrient Requirements of Laboratory Animals, 3rd ed”, p.23. National Academy of Science, Washington, DC (1978).Google Scholar
  26. 26.
    D. J. Knauer and G. L. Smith, Inhibition of biological activity of multiplication-stimulating activity by binding to its carrier protein. Proc. Natl. Acad. Sci. USA. 77:7252 (1980).PubMedCrossRefGoogle Scholar
  27. 27.
    O. Ritvos, T. Ranta, J. Jalkanen, A.-M. Suikkari, R. Voutilainen, H. Bohn, and E.-M. Rutanen, Insulin-like growth factor (IGF) binding protein from human decidua inhibits the binding and biological action of IGF-I in cultured chondriocarcinoma cells. Endocrinology 122:2150 (1988).PubMedCrossRefGoogle Scholar
  28. 28.
    R. G. Elgin, W. H. Busby, Jr. and D. R. Clemmons, An insulin-like growth factor (IGF) binding protein enhances the biologic response to IGF-I. Proc. Natl. Acad. Sci. USA. 84:3254 (1987).PubMedCrossRefGoogle Scholar
  29. 29.
    W. H. Busby, P. Hossenlopp, M. Binoux, and D. R. Clemmons, Purified preparations of the amniotic fluid-derived insulin-like growth factor-binding protein contain multimeric forms that are biologically active. Endocrinology 125:773 (1989).PubMedCrossRefGoogle Scholar
  30. 30.
    W. F. Blum, E. W. Jenne, F. Reppin, K, Kietzmann, M. B. Ranke, and J. R. Bierich, Insulin-like growth factor I (IGF-I)-binding protein complex is a better mitogen than free IGF-I. Endocrinology 125:766 (1989).PubMedCrossRefGoogle Scholar
  31. 31.
    J. S. M. DeMellow, and R. C. Baxter, Growth hormone-dependent insulinlike growth factor (IGF) binding protein both inhibits and potentiates IGF-I stimulated DNA synthesis in human skin fibroblasts. Biochem. Biophys. Res. Commun. 156:199 (1988).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Hisanori Kato
    • 1
  • Tsutomu Umezawa
    • 1
  • Yutaka Miura
    • 1
  • Tadashi Noguchi
    • 1
  1. 1.Department of Agricultural Chemistry, Faculty of AgricultureThe University of TokyoBunkyo-ku, Tokyo 113Japan

Personalised recommendations