Advertisement

Cerebrospinal IGF Binding Proteins : Isolation and Characterization

  • Michel Binoux
  • Monireh Roghani
  • Paul Hossenlopp
  • Odile Whitechurch
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 293)

Abstract

In biological fluids, IGFs (insulin-like growth factors I and II) are associated with specific, high-affinity (≈ 1010 M-1) binding proteins (BPs) which control both their bioavailability and their action at target cell level (Review in 1). Research done in our laboratory has shown that the BPs are molecularly and functionally heterogenous. Using Western ligand blotting, we have identified five molecular forms in man, of 41.5, 38.5, 34, 30 and 24 kDa. The proportions of these forms vary in different biological fluids and culture media and their regulation and affinities for IGF-I and IGF-II are different (2–6).

Keywords

Amniotic Fluid Human Amniotic Fluid Selective Affinity Target Cell Level Western Ligand Blot Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Baxter RC, Martin JL 1989 Binding proteins for the insulin-like growth factors : structure, regulation and function. Progress in Growth Factor Research 1:49–68.PubMedCrossRefGoogle Scholar
  2. 2.
    Binoux M, Hardouin S, Lassarre C, Hossenlopp P 1982 Evidence for production by the liver of two IGF binding proteins with similar molecular weights but different affinities for IGF I and IGF II. Their relations with serum and cerebrospinal fluid IGF binding proteins. J Clin Endocrinol Metab 55:600–602.PubMedCrossRefGoogle Scholar
  3. 3.
    Hossenlopp P, Seurin D, Segovia-Quinson B, Binoux M 1986 Identification of an insulin-like growth factor binding protein in human cerebrospinal fluid with a selective affinity for IGF II. FEBS Lett 208:439–444.PubMedCrossRefGoogle Scholar
  4. 4.
    Hardouin S, Hossenlopp P, Segovia B, Seurin D, Portolan G, Lassarre C, Binoux M 1987 Heterogeneity of insulin-like growth factor binding proteins and relationships between structure and affinity. 1. Circulating forms in man. Eur J Biochem 170 : 121– 132.PubMedCrossRefGoogle Scholar
  5. 5.
    Hossenlopp P, Seurin D, Segovia B, Portolan G, Binoux M 1987 Heterogeneity of insulin-like growth factor binding proteins and relationships between structure and affinity. 2. Forms released by human and rat liver in culture. Eur J Biochem 170 : 133– 142.PubMedCrossRefGoogle Scholar
  6. 6.
    Hardouin S, Gourmelen M, Noguiez P, Seurin D, Roghani M, Le Bouc Y, Povoa G, Merimee TJ, Hossenlopp P, Binoux M 1989 Molecular forms of serum insulin-like growth factor (IGF) binding proteins in man : relationships with growth hormone and IGFs and physiological significance. J Clin Endocrinol Metab 69: 1291–1301.PubMedCrossRefGoogle Scholar
  7. 7.
    Martin JL, Baxter RC 1986 Insulin-like growth factor-binding protein from human plasma. Purification and characterization. J Biol Chem 261:8754–8760.PubMedGoogle Scholar
  8. 8.
    Wood WI, Cachianes G, Henzel WJ, Winslow GA, Spencer SA, Hellmiss R, Martin JL, Baxter RC 1988 Cloning and expression of the growth hormone-dependent insulinlike growth factor-binding protein. Molecular Endocrinology 2: 1176–1185.PubMedCrossRefGoogle Scholar
  9. 9.
    Ballard J, Baxter R, Binoux M, Clemmons D, Drop S, Hall K, Hintz R, Rechler M, Rutanen E, Schwander J 1989 On the nomenclature of the IGF binding proteins. Acta Endocrinol (Copenh) 121:751–752.Google Scholar
  10. 10.
    Binkert C, Landwehr J, Mary JL, Schwander J, Heinrich G 1989 Cloning, sequence analysis and expression of a cDNA encoding a novel insulin-like growth factor binding protein (IGF BP-2). EMBO J 8:2497–2502.PubMedGoogle Scholar
  11. 11.
    Zapf J, Kiefer M, Merryweather J, Masiarz F, Bauer D, Born W, Fischer J A, Froesch ER 1990 Isolation from adult human serum of four insulin-like growth factor (IGF) binding proteins and molecular cloning of one of them that is increased by IGF I administration and in extrapancreatic tumor hypoglycemia. J Biol Chem (in press).Google Scholar
  12. 12.
    Drop SLS, Kortleve DJ, Guyda HJ 1984 Isolation of a somatomedin-binding protein from preterm amniotic fluid. Development of a radioimmunoassay. J Clin Endocrinol Metab 59:899–907.PubMedCrossRefGoogle Scholar
  13. 13.
    Povoa G, Enberg G, Jornvall H, Hall K 1984 Isolation and characterization of a somatomedin-binding protein from mid-term human amniotic fluid. Eur J Biochem 144:199–204.PubMedCrossRefGoogle Scholar
  14. 14.
    Povoa G, Isaksson M, Jörnvall H, Hall K 1985 The somatomedin-binding protein isolated from a human hepatoma cell line is identical to the human amniotic fluid somatomedin-binding protein. Biochem Biophys Res Commun 128: 1071–1078.PubMedCrossRefGoogle Scholar
  15. 15.
    Brewer MT, Stetler GL, Squires CH, Thompson RC, Busby WH, Clemmons DR 1988 Cloning, characterization, and expression of a human insulin-like growth factor binding protein. Biochem Biophys Res Comm 152:1289–1297.PubMedCrossRefGoogle Scholar
  16. 16.
    Brinkman A, Groffen C, Kortleve DJ, Geurts van Kessel A, Drop SLS 1988 Isolation and characterization of a cDNA encoding the low molecular weight insulin-like growth factor binding protein (IBP-1). EMBO J 7:2417–2423.PubMedGoogle Scholar
  17. 17.
    Julkunen M, Koistinen R, Aalto-Setälä K, Seppälä M, Jänne OA, Kontula K 1988 Primary structure of human insulin-like growth factor-binding protein/placental protein 12 and tissue-specific expression of its mRNA. FEBS Lett 236:295–302.PubMedCrossRefGoogle Scholar
  18. 18.
    Lee YL, Hintz RL, James PM, Lee PDK, Shively JE, Powell DR 1988 Insulin-like growth factor (IGF) binding protein complementary deoxyribonucleic acid from human Hep G2 hepatoma cells : predicted protein sequence suggests an IGF binding domain different from those of the IGF-I and IGF-II receptors. Mol Endocrinol 2:404–411.PubMedCrossRefGoogle Scholar
  19. 19.
    Mohan S, Bautista CM, Wergedal J, Baylink DJ 1989 Isolation of an inhibitory insulin-like growth factor (IGF) binding protein from bone cell-conditioned medium : a potential local regulator of IGF action. Proc Natl Acad Sci USA 86:8338–8342.PubMedCrossRefGoogle Scholar
  20. 20.
    Haselbacher G, Humbel R 1982 Evidence for two species of insulin-like growth factor II (IGF II and “big” IGF II) in human spinal fluid. Endocrinology 110: 1822–1824.PubMedCrossRefGoogle Scholar
  21. 21.
    Roghani M, Hossenlopp P, Lepage P, Balland A, Binoux M 1989 Isolation from human cerebrospinal fluid of a new insulin-like growth factor binding protein with a selective affinity for IGF II. FEBS Lett 255:253–258.PubMedCrossRefGoogle Scholar
  22. 22.
    Roghani M, Lassarre C, Zapf J, Povoa G, Binoux M Two insulin-like growth factor binding proteins are responsible for the selective affinity for IGF-II of cerebrospinal fluid binding proteins. J Clin Endocrinol Metab (in press).Google Scholar
  23. 23.
    Lamson G, Pham H, Oh Y, Ocrant I, Schwander J, Rosenfeld RG 1989 Expression of the BRL-3A Insulin-like growth factor binding protein (rBP-30) in the rat central nervous system. Endocrinology 123: 1100–1102.CrossRefGoogle Scholar
  24. 24.
    Martin JL, Willetts KE, Baxter RC 1990 Purification and properties of a novel insulinlike growth factor-II binding protein from transformed human fibroblasts. J Biol Chem 265:4124–4130.PubMedGoogle Scholar
  25. 25.
    Zapf J, Born W, Chang JY, James P, Froesch ER, Fischer JA 1988 Isolation and NH2-terminal amino acid sequences of rat serum carrier proteins for insulin-like growth factors. Biochem Biophys Res Commun 156: 1187–1194.PubMedCrossRefGoogle Scholar
  26. 26.
    Hossenlopp P, Segovia B, Lassarre C, Roghani M, Bredon M, Binoux M Evidence of enzymatic degradation of insulin-like growth factor binding proteins in the “150 K” complex during pregnancy. J Clin Endocrinol Metab (in press).Google Scholar
  27. 27.
    Shimonaka M, Schroeder R, Shimasaki S, Ling N 1989 Identification of a novel binding protein for insulin-like growth factors in adult rat serum. Biochem Biophys Res Commun 165:189–195.PubMedCrossRefGoogle Scholar
  28. 28.
    Baxter RC, Martin JL 1987 Binding proteins for insulin-like growth factors in adult rat serum. Comparison with other human and rat binding proteins. Biochem Biophys Res Commun 147:408–415.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Michel Binoux
    • 1
  • Monireh Roghani
    • 1
  • Paul Hossenlopp
    • 1
  • Odile Whitechurch
    • 2
  1. 1.INSERM U. 142Hôpital Saint-AntoineParisFrance
  2. 2.Transgène S.A.StrasbourgFrance

Personalised recommendations