γδ T Cells in Murine Epithelia: Origin, Repertoire, and Function

  • James P. Allison
  • David M. Asarnow
  • Mark Bonyhadi
  • Amy Carbone
  • Wendy L. Havran
  • Diphankar Nandi
  • Janelle Noble
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 292)


The overwhelming majority of T cells in the thymus and peripheral lymphoid organs express an antigen receptor (TCR) comprised of αβ heterodimers in association with the CD3 complex. The repertoire of αβ T cells is shaped for the recognition of peptide antigens in the context of MHC Class I or Class II antigens by cells bearing CD8 or CD4, respectively. A minor population of T cells in the lymphoid organs express TCR composed of δ heterodimers, and these cells typically do not express CD4, although a small fraction expresses CD8.1 In certain epithelial tissues of the mouse, however, γδ T cells comprise the bulk, if not the entirety, of the T cells. There is a tight correlation between Vγ gene usage and epithelial localization as well as differences in the potential TCR repertoire in the different epithelia, two features which raise important questions as to the origin, homing, selection, and function of these cells.


Reproductive Tract Polymerase Chain Reac Intraepithelial Lymphocyte Versus Segment Junctional Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    D. H. Raulet, The structure, function, and molecular genetics of the 8 T cell receptor, Ann. Rev. Immunol. 7:175 (1989).CrossRefGoogle Scholar
  2. 2.
    R. D. Garman, P. J. Doherty, and D. H. Raulet, Diversity, rearrangement, and expression of murine T cell 7 genes, Cell 45:733 (1986).PubMedCrossRefGoogle Scholar
  3. 3.
    Y. H. Chien, M. Iwashima, D. A. Wettstein, K. B. Kaplan, J. F. Elliott, W. Born, and M. M. Davis, T-cell receptor 8 gene rearrangements in early thymocytes, Nature 330:722 (1987).PubMedCrossRefGoogle Scholar
  4. 4.
    W. L. Havran and J. P. Allison, Developmental ordered appearance of thymocytes expressing different T cell antigen receptors, Nature 335:443 (1988).PubMedCrossRefGoogle Scholar
  5. 5.
    S. Itohara, N. Nakanishi, O. Kanagawa, R. Kubo, and S. Tonegawa, Monoclonal antibodies specific to native murine T-cell receptor Analysis of T cells during thymic ontogeny and in peripheral lymphoid organs, Proc. Natl. Acad. Sci. USA 86:5094 (1989).PubMedCrossRefGoogle Scholar
  6. 6.
    W. A. Kuziel, A. Takashima, M. Bonyhadi, P. R. Bergstresser, J. P. Allison, R. E. Tigelaar, and P. W. Tucker, Regulation of T-cell receptor -chain RNA expression in murine Thy-1+ dendritic epidermal cells, Nature 328:263 (1987).PubMedCrossRefGoogle Scholar
  7. 7.
    W. L. Havran, S. C. Grell, G. Duwe, J. Kimura, A. Wilson, A. M. Kruisbeek, R. L. O’Brien, W. Born, R. E. Tigelaar, and J. P. Allison, Limitied diversity of TCR chain expression of murine Thy-1+ dendritic epidermal cells revealed by V3-specific monoclonal antibody, Proc. Natl. Acad. Sci. USA 86:4185 (1989).PubMedCrossRefGoogle Scholar
  8. 8.
    D. M. Asarnow, W. A. Kuziel, M. Bonyhadi, R. E. Tigelaar, P. W. Tucker, and J. P. Allison, Limited diversity of antigen receptor genes of Thy-1+ dendritic epidermal cells, Cell 55:837 (1988).PubMedCrossRefGoogle Scholar
  9. 9.
    D. Asarnow, T. Goodman, L. LeFrancois, and J. P. Allison, Distinct antigen receptor repertoires of two classes of murine epithelium-associated T cells, Nature 341:60 (1989).PubMedCrossRefGoogle Scholar
  10. 10.
    T. Goodman and L. LeFrancois, Expression of the gamma/delta T-cell receptor on intestinal CD8+ intraepithelial lymphocytes, Nature 333:855 (1988).PubMedCrossRefGoogle Scholar
  11. 11.
    M. Bonneville, C. A. Janeway, Jr., K. Ito, W. Haser, I. Ishida, N. Nakanishi, and S. Tonegawa, Intestinal intraepithelial lymphocytes are a distinct set of 8 T cells, Nature 336:479 (1988).PubMedCrossRefGoogle Scholar
  12. 12.
    S. Itohara, A. G. Farr, J. J. Lafaille, M. Bonneville, Y. Takagaki, W. Haas, and S. Tonegawa, Homing of a thymocyte subset with homogenous T-cell receptors to mucosal epithelia, Nature 343:754 (1990).PubMedCrossRefGoogle Scholar
  13. 13.
    P. R. Bergstresser, S. Sullivan, J. W. Streilein, and R. F. Tigelaar, Origin and function of Thy-1+ dendritic epidermal cells in mice, J. Invest. Derm. 85:85 (1985).CrossRefGoogle Scholar
  14. 14.
    W. L. Havran and J. P. Allison, Origin of Thy-1+ dendritic epidermal cells of adult mice from fetal thymic precursors, Nature 344:68 (1990).PubMedCrossRefGoogle Scholar
  15. 15.
    J. L. Nixon-Fulton, W. A. Kuziel, B. Santerse, P. R. Bergstresser, P. W. Tucker, and R. E. Tigelaar, Thy-1+ epidermal cells in nude mice are distinct from their counterparts in thymus-bearing mice. A study of morphology, function, and T cell receptor expression, J. Immunol. 141:1897 (1988).PubMedGoogle Scholar
  16. 16.
    M. Iwashima, A. Green, M. Bonyhadi, M. M. Davis, J. P. Allison, and Y.-H. Chien, Thymic and post-thymic selection of T cells in T cell receptor transgenic mice. Submitted.Google Scholar
  17. 17.
    M. M. Davis and P. J. Bjorkman, T cell antigen receptor genes and T cell recognition, Nature 334:395 (1988).PubMedCrossRefGoogle Scholar
  18. 18.
    J. J. Lafaille, A. DeCloux, M. Bonneville, Y. Takagaki, and S. Tonegawa, Junctional sequences of T cell receptor 8 genes: Implications for T cell lineages and for a novel intermediate of V-(D)-J joining, Cell 59:859 (1990).CrossRefGoogle Scholar
  19. 19.
    R. A. Young and T. J. Elliott, Stress proteins, infection, and immune surveillance, Cell 59:5 (1989).Google Scholar
  20. 20.
    W. Born, M. P. Happ, A. Dallas, C. Reardon, R. Kubo, T. Shinnick, P. Brennan, and R. O’Brien, Recognition of heat shock proteins and gamma/delta cell function, Immunol. Today 11:40 (1990).PubMedCrossRefGoogle Scholar
  21. 21.
    C. A. Smith, G. T. Williams, R. Kingston, E. J. Jenkinson, and J. J. T. Owen, Antibodies to CD3/T-cell receptor complex induce death by apoptosis in immature T cells in thymic cultures, Nature 337:181 (1989).PubMedCrossRefGoogle Scholar
  22. 22.
    B. J. Fowlkes and D. M. Pardoll, Molecular and cellular events of T cell development, Adv. Immunol 44:207 (1989).PubMedCrossRefGoogle Scholar
  23. 23.
    M. K. Jenkins, R. H. Schwartz, and D. M. Pardoll, Effects of cyclosporine A on T cell development and clonal deletion, Science 241:1655 (1988).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • James P. Allison
    • 1
  • David M. Asarnow
    • 1
  • Mark Bonyhadi
    • 1
  • Amy Carbone
    • 1
  • Wendy L. Havran
    • 1
  • Diphankar Nandi
    • 1
  • Janelle Noble
    • 1
  1. 1.Division of Immunology, Department of Molecular and Cell Biology, and Cancer LaboratoryUniversity of CaliforniaBerkeleyUSA

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