First Wave Fetal Thymocytes Expressing V3Jγ1Cγ1-Vδ1Jδ2Cδ T Cell Receptors are not Required For αβ T Cell Receptor Rearrangement and Expression
We have determined the fetal expression of Vγ3, δ and β TcRs in mice transgenic for the Vγ1.lJ4Cγ4 TcR chain. The first wave thymocytes appearing at day 14 and disappearing by day 17 in normal mice was absent from the transgenic mice. However, both mice had an almost identical number of γδ-bearing thymocytes throughout gestation. Therefore, it is most likely that the V73J7IC7I chain was replaced in the transgenic mice by the Vγ1.1Jγ4Cγ4 transgene. The appearance, although slightly earlier for the transgenic mice, of αβ-bearing thymocytes was also very similar between transgenic and control mice during gestation. These data suggest that whatever the role of the first wave thymocytes expressing V3-Vδ1 TcRs is, it most likely is not required for the rearrangement, expression and maturation of the aβ TcR repertoire. We are currently analyzing a series of γδ transgenic mice to determine whether other restricted populations of γδ-bearing T cells are involved in specific aspects of immune development or function.
KeywordsTransgenic Mouse Fetal Thymus Antigen Receptor Gene Dendritic Epidermal Cell Fetal Gestation
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