Stromal Cell Lines which Support Lymphocyte Growth
Cloned stromal cell lines developed by a number of laboratories are revealing complexity in the hemopoietic inductive microenvironment.1,2 From these, we now know a considerable amount about genes that may be constitutively expressed in stromal cells and those whose expression is elicited by exogenous stimuli. It is also becoming obvious that stromal cells retain differentiation potential, with a striking example being their differentiation to adipocytes.3 However, no single stromal cell clone supports all the B lineage differentiation events which normally occur within bone marrow and there are indications that stromal cells in culture differ in some ways from their counterparts in situ. Most stromal cell lines which have been described were originally selected for their ability to support growth and/or differentiation of a particular type of hemopoietic cell. Our BMS2 clone permits continuous growth of a number of cloned lymphocytes, which were themselves derived from long term bone marrow cultures.4 Subclones of BMS2 were prepared and analyzed to determine how stable the line is with repeated passage and if any variations in function correspond to expression of known genes. We now describe one subclone with several unique characteristics which antagonizes lymphocyte growth.
KeywordsStromal Cell Murine Bone Marrow Stromal Cell Line Exogenous Stimulus Adherent Layer
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