Cloning and Characterization of a Protein Binding to the Jκ Recombination Signal Sequence of Immunoglobulin Genes
A protein with molecular weight of 60,000 that binds to the recombination signal sequence (RS) of the immunoglobulin Jκ segment was purified from the nuclear extract of a murine pre B cell line 38B9. This binding protein was found in lymphoid cell lines but not in non-lymphoid cell lines. The Kd value of the Jκ RS binding protein to the Jκ RS was 1 nM. The cDNA clone (RBP-2) was isolated based on partial amino-acid sequence of this protein. This cDNA encodes 526 amino-acid residues, and its sequence does not show extensive overall homology with any known proteins, but displays an interesting homology to a 40-residue region that is conserved among a subset of site specific recombinase (integrase family).
KeywordsImmunoglobulin Gene Partial Amino Acid Sequence Lymphoid Cell Line Variable Region Gene Cell Receptor Gene
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