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Cloning and Characterization of a Protein Binding to the Jκ Recombination Signal Sequence of Immunoglobulin Genes

  • Yasushi Hamaguchi
  • Norisada Mastunami
  • Yoshiki Yamamoto
  • Kogo Kuze
  • Kenji Kangawa
  • Hisayuki Matsuo
  • Masashi Kawaichi
  • Tasuku Honjo
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 292)

Summary

A protein with molecular weight of 60,000 that binds to the recombination signal sequence (RS) of the immunoglobulin Jκ segment was purified from the nuclear extract of a murine pre B cell line 38B9. This binding protein was found in lymphoid cell lines but not in non-lymphoid cell lines. The Kd value of the Jκ RS binding protein to the Jκ RS was 1 nM. The cDNA clone (RBP-2) was isolated based on partial amino-acid sequence of this protein. This cDNA encodes 526 amino-acid residues, and its sequence does not show extensive overall homology with any known proteins, but displays an interesting homology to a 40-residue region that is conserved among a subset of site specific recombinase (integrase family).

Keywords

Immunoglobulin Gene Partial Amino Acid Sequence Lymphoid Cell Line Variable Region Gene Cell Receptor Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Yasushi Hamaguchi
    • 1
  • Norisada Mastunami
    • 1
  • Yoshiki Yamamoto
    • 1
  • Kogo Kuze
    • 1
  • Kenji Kangawa
    • 2
  • Hisayuki Matsuo
    • 2
  • Masashi Kawaichi
    • 1
  • Tasuku Honjo
    • 1
  1. 1.Department of Medical Chemistry, Faculty of MedicineKyoto UniversityKyotoJapan
  2. 2.Department of BiochemistryMiyazaki Medical CollegeMiyazakiJapan

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