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Expression of Receptors for Interleukin 4 and Interleukin 7 on Human T Cells

  • Richard J. Armitage
  • Steven F. Ziegler
  • M. Patricia Beckmann
  • Rejean L. Idzerda
  • Linda S. Park
  • William C. Fanslow
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 292)

Summary

Human recombinant interleukin 4 (IL-4) and interleukin 7 (IL-7) have been modified with biotin-N-hydroxysuccinimide and used to examine the expression of human IL-4 and IL-7 receptors (R) on activated peripheral blood T cells by flow cytometry. Freshly isolated T cells expressed only a low level of IL-4R which remained unchanged when cells were cultured in the absence of stimuli. In the presence of IL-4, IL-7, phytohemag-glutinin A (PHA) or immobilized CD3 monoclonal antibody the intensity of biotinylated IL-4 staining increased approximately twofold on the majority of cells. A combination of mitogen with either IL-4 or IL-7 caused a considerable increase in IL-4 receptor expression over that seen in the presence of mitogen alone. IL-2 alone failed to induce IL-4R although it was able to cause a significant increase in receptor expression on T cells co-cultured with PHA or CD3.

Freshly isolated T cells expressed high levels of IL-7R, as determined by biotinylated IL-7 binding and flow cytometry, which did not change significantly with culture in medium alone. Stimulation with PHA, Concanavalin A (Con A) or CD3 had little effect on the intensity of staining. In contrast, activation with phorbol ester resulted in a decrease in IL-7R expression. Similarly, in the presence of IL-4 or IL-7, but not IL-2, the intensity of staining with biotinylated IL-7 was lowered. Analysis of purified T-cell populations showed that IL-7R were present, and IL-4R could be induced, on both CD4+ and CD8+ populations.

Analysis of IL-4 receptor expression by this flow cytometric technique was supported by results from 125I-labeled IL-4 binding and by Northern blot analysis of mRNA levels. Taken together, the results of these studies show that the use of biotinylated cytokines and flow cytometry provides a very sensitive method with which to study the expression and regulation of cytokine receptors.

Keywords

Mean Fluorescence Intensity Cell Stimulatory Factor Flow Cytometric Technique Murine Lymph Node Real Biological Difference 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Richard J. Armitage
    • 1
  • Steven F. Ziegler
    • 1
  • M. Patricia Beckmann
    • 1
  • Rejean L. Idzerda
    • 1
  • Linda S. Park
    • 1
  • William C. Fanslow
    • 1
  1. 1.Immunex CorporationSeattleUSA

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