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Characterization of Triple Negative Clones Isolated from Post-Natal Human Thymus

  • Toshiyuki Hori
  • Hergen Spits
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 292)

Abstract

Human triple negative (CD3- CD4- CD8-) thymocytes and double negative (CD4- CD8-) thymocytes purified from post-natal thymus were cloned with a feeder cell mixture of irradiated PBL, irradiated JY cells and PHA and expanded with IL-2. The cloning efficiency of triple negative thymocytes was less than 1% and the majority of the clones were triple negative. One out of 11 clones was TCR αβ+ CD4+. No TCR γδ + clones were isolated. On the other hand, the cloning efficiency of double negative thymocytes was about 10% and most of the clones isolated were TCR γδ +. We could not find any evidence of in vitro differentiation of triple negative thymocytes into TCR γδ + cells. Some of the triple negative clones expressed CD16- brightly and were apparently NK cells. All CD 16-clones isolated from triple negative thymocytes, however, expressed NKH1, which is also an NK cell marker. Cytoplasmic CD3-δ and CD3-ε Ag which have been reported to be expressed in the most immature thymocytes were not detected in any of these clones. Furthermore, the CD 16- triple negative clones exhibited significant cytolytic activity against K562. Pheno-type of the clones seems to be stable under various conditions in vitro including coculture with human thymic epithelial cells. These data indicate that the CD16- triple negative clones isolated from triple negative thymocytes are similar to a minor subset of NK cells which is CD16- NKH-1+. It is not clear whether they originated from a distinct subset of mature or immature NK cells resident in the thymus tissue or from common precursors of both T and NK lineage.

Keywords

Cloning Efficiency Thymus Tissue Human Thymus Immature Thymocyte Thymic Stroma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1991

Authors and Affiliations

  • Toshiyuki Hori
    • 1
  • Hergen Spits
    • 1
  1. 1.The Department of Human ImmunologyDNAX Research Institute of Molecular and Cellular ImmunologyPalo AltoUSA

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