Cultured Human Placental Trophoblasts as Models for Investigating Defective Regulation of Chloride Transport and Protein Secretion in Cystic Fibrosis

  • M. P. Ryan
  • C. Somers
  • M. X. Fitzgerald
  • A. K. Keenan
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 290)

Abstract

Human placenta provides a major source of epithelial material for investigating basic defects in cystic fibrosis (CF). The placenta has high transport capabilities, contains the same genotype as the foetus and is known to express the CF gene. The present study concerns the establishment of a primary cell culture system for human placental trophoblasts and characterisation of chloride transport and gonadotrophin secretion as models for investigating CF defects.

Keywords

EDTA Penicillin Trypsin Streptomycin Nylon 

References

  1. Contractor, S.F. and Sooranna, S.R., 1988, J. Devl. Physiol., 10:47.Google Scholar
  2. Kliman, H.J., Nestler, J.E., Sermasi, E., Sanger, J.M. and Strauss, J.F., 1986, Endocrinology, 118:1567. 392PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • M. P. Ryan
    • 1
  • C. Somers
    • 1
  • M. X. Fitzgerald
    • 1
  • A. K. Keenan
    • 1
  1. 1.Departments of Pharmacology and MedicineUniversity CollegeDublinIreland

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