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Study of Reconstitution of the Rabbit Parotid Na+/K+/2Cl-Cotransporter

  • Concetta Ferri
  • Monica Garnier
  • Angela Corcelli
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 290)

Abstract

The critical role of Cl- transepithelial transport in eliciting a normal fluid secretion or absorption in various epithelia is strongly suggested from clinical and pathological studies. Modifications of epithelial function caused from bacterial toxins or genetically determined, as in cystic fibrosis, appear to be specifically dependent on some alteration in Cl- transport across epithelia. The apical and basolateral membranes of epithelial cells are endowed with Cl- transport systems of different specificities and characteristics. According to the current view on ion transepithelial transport, the entry of Cl- into the cell can occur via cation coupled transport systems. In many absorbing and secreting epithelia a quaternary cotransport system of stoichiometry lNa+: 1K+:2C1- has been characterized. In order to understand the mechanism of transport and ion coupling of such complex transport system, it is important to have it in a functional state in reconstituted systems. In this type of study the availability of an high affinity inhibitor of the transporter can provide a useful tool to label the protein in various steps of solubilization and reconstitution. As regards the Na+/K+/2Cl- cotransporter the appropriate labelling agent to use for this purpose appears to be bumetanide.

Keywords

Cystic Fibrosis Basolateral Membrane Transepithelial Transport Reconstituted System Labelling Agent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Corcelli, A., Turner, R.J., 1990, Reconstitution of the rabbit parotid basolateral membrane Na/K/Cl dependent bumetanide binding site, Annals New York Academy of Sciences, 574:106.CrossRefGoogle Scholar
  2. 2.
    Turner, R.J., George, J.N., 1988, Ionic dependence of bume-tanide binding to the rabbit parotid Na/K/Cl cotransporter, J. Membrane Biol., 102:71.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Concetta Ferri
    • 1
  • Monica Garnier
    • 1
  • Angela Corcelli
    • 1
  1. 1.Istituto di Fisiologia GeneraleUniversita’ di BariItaly

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