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DNA Analysis in CF Families by Biotinylated Probes and Polymerase Chain Reaction Technique

  • C. Camporese
  • L. Picci
  • N. A. Greggio
  • M. Abdol Mohammady
  • A. Barbato
  • L. Chiandetti
  • F. Anglani
  • F. Zacchello
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 290)

Abstract

About 70% of the mutations in the cystic fibrosis (CF) gene in North American, British and Dutch patients are caused by a 3 bp deletion referred to as DeltaF508 (Kerem et al., 1989).

Keywords

Cystic Fibrosis Prenatal Diagnosis Polymerase Chain Reaction Technique Informative Pattern Carrier Detection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Anglani, F., Camporese, C., Greggio, N.A., Murgia, A., Zacchel-lo, F., 1990, The use of biotinylated probes in the DNA analysis for diagnostic purpose, CHIMICAoggi, Inter. J. of Chem. and Biotech., (in press).Google Scholar
  2. Kerem, B.S., Rommens, J., Duchanan, H., Markiewicz, D., Cox, T., Chakravarti, A., Buchwald, M., et al., 1989, Identification of the cystic fibrosis gene: genetic analysis. Science, 245:1073.PubMedCrossRefGoogle Scholar
  3. Mathew, C.G. ,Roberts, R.G., Harris, A., Bentley, D.P., Bobrow, M., 1989, Rapid screening for DeltaF508 deletion in Cystic Fibrosis, Lancet, II:1346.Google Scholar
  4. Estivill, X., Gasparini, F., Novelli, G., Benitez, E.F.J., Williamson, P., et al., 1989a, DeltaF508 gene deletion in cystic fibrosis in southern Europe, Lancet, ii:1404.CrossRefGoogle Scholar
  5. Estivill, X., Gasparini, P., Novelli G., Casals T., Nunes V., Gallano, P., Savoia, A., Ruzzo, A., Dallapiccola B., Pignat-ti, P.F., 1989b, Linkage disequilibrium for DNA haplotypes near the cystic fibrosis locus in two South European populations, Hum. Genet., 83:175.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • C. Camporese
    • 1
  • L. Picci
    • 1
  • N. A. Greggio
    • 1
  • M. Abdol Mohammady
    • 1
  • A. Barbato
    • 1
  • L. Chiandetti
    • 1
  • F. Anglani
    • 1
  • F. Zacchello
    • 1
  1. 1.Dept. of PediatricsUniversity of PadovaItaly

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