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The CF Gene Product as a Member of a Membrane Transporter (TM6-NBF) Super Family

  • John R. Riordan
  • Noa Alon
  • Zbyszko Grzelczak
  • Steve Dubel
  • Shi-Zhang Sun
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 290)

Abstract

Since the isolation of the CF gene, efforts have been underway to determine the specific function of its protein product. Properties of the molecule inferred from its predicted primary structure (1) provided relatively strong biases as to its likely function. Hence, the presence of several sequence segments, capable of forming hydrophobic alpha helices of appropriate length to cross a lipid bilayer, suggests strongly that it is an integral membrane protein. The existence of hydrophilic domains with similarity to nucleotide-binding sequences of other membrane proteins involved in transport implies that CFTR itself could be a transporter. Of course, at this time, such a suggestion is only conjecture. To reach beyond this stage, functional studies employing antibodies to the protein and expressible cDNAs must be carried out. In the meantime, however, it is instructive to evaluate CFTR from the points of view of the known functions of related members of the superfamily and its possible relation to current models of epithelial ion transport. The need to undertake the latter considerations is emphasized by the fact that it is strongly expressed in tissues which are highly specialized to perform active salt secretion.

Keywords

Super Family Hydrophilic Domain Rectal Gland Photoaffinity Labelling Cystic FibroSIS 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • John R. Riordan
    • 1
    • 2
  • Noa Alon
    • 1
    • 2
  • Zbyszko Grzelczak
    • 1
    • 2
  • Steve Dubel
    • 1
    • 2
  • Shi-Zhang Sun
    • 1
    • 2
  1. 1.The Hospital for Sick ChildrenTorontoCanada
  2. 2.The University of TorontoTorontoCanada

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