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A Chloride Conductance Evoked by Hypotonic Shock in Epithelial Cells

  • L. J. V. Galietta
  • V. Barone
  • D. C. Gruenert
  • G. Romeo
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 290)

Abstract

Single channel recordings have shown that airway epithelial cells possess apical rectifying Cl- channels whose activity is regulated by the cAMP pathway. Several reports (Frizzell et al., 1986; Welsh and Liedtke 1986; Schoumacher et al., 1987; Li et al., 1988) indicate an altered regulation of these chloride channels as the possible functional defect causing cystic fibrosis (CF). We asked whether the defective regulation of Cl- channels in CF can also be detected in the whole-cell configuration of the patch-clamp technique. This approach, which allows to record macroscopic membrane currents, gives a more representative view of the whole cell membrane conductance. The possible existence of different types of Cl- currents can be more easily detected. Furthermore, in polarized cells, the whole-cell technique allows to record currents flowing through the basolateral membrane which is not accessible to the patch pipette. Recent reports using the whole-cell approach have shown that colonic carcinoma T84 cells (Worrell et al., 1989) and primary culture respiratory cells (Schoppa et al., 1989; McCann et al., 1989) have a Cl- conductance characterized by inactivation at positive membrane potentials. Two of these papers (Worrell et al., 1989; McCann et al., 1989) report that the inactivating Cl- current is sensitive to cell volume changes.

Keywords

Cystic Fibro Chloride Channel Chloride Current Tracheal Epithelial Cell Chloride Conductance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • L. J. V. Galietta
    • 1
  • V. Barone
    • 1
  • D. C. Gruenert
    • 2
  • G. Romeo
    • 1
  1. 1.Lab. di Genetica MolecolareIst. G. GasliniGenovaItaly
  2. 2.Cardiovascular Research InstituteUniversity of CaliforniaSan FranciscoUSA

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